Fluorescent Rna Assay
Fluorescent Rna Assay - Related Videos
09:57
Whole Mount RNA Fluorescent in situ Hybridization of Drosophila Embryos
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Cited by 22 •
2013
Here we describe a whole-mount fluorescent in situ hybridization (FISH) protocol for determining the expression and localization properties of RNAs expressed during embryogenesis in the fruit fly, Drosophila melanogaster.
10:16
Detection of Viral RNA by Fluorescence in situ Hybridization (FISH)
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Cited by 35 •
2012
A fluorescence in situ hybridization (FISH) method was developed to visually detect viral genomic RNA using fluorescence microscopy. A probe is made with specificity to the viral RNA that can then be identified using a combination of hybridization and immunofluorescence techniques. This technique offers the advantage of identifying the localization of the viral RNA or DNA at steady-state, providing information on the control of intracellular virus trafficking events.
05:52
Rapid Colorimetric Assays to Qualitatively Distinguish RNA and DNA in Biomolecular Samples
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Cited by 22 •
2013
A suite of colorimetric assays is described for rapidly distinguishing protein, RNA, DNA, and reducing sugars in potentially heterogeneous biomolecular samples.
05:07
RNA-Protein Pull-Down Assay to Isolate RNA-Binding Proteins via Affinity Extraction
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2025
This video demonstrates an in vitro RNA pull-down assay to identify RNA-binding proteins (RBPs), which interact with the adenylate-uridylate-rich element (ARE) sequences in mRNA. The target RBPs from a cell lysate are mixed with an RNA probe to form RNA-protein complexes. The complexes are isolated via affinity purification utilizing the affinity of the desthiobiotin label of the RNA probe to a streptavidin-labeled magnetic bead.
02:59
Using Immuno-RNA Fluorescence In Situ Hybridization to Visualize SARS-CoV-2
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2025
This video demonstrates the application of Hybridization Chain Reaction (HCR)-Fluorescence In Situ Hybridization (FISH) for visualizing the spatial distribution of SARS-CoV-2 subgenomic RNA. Combining HCR with immunofluorescence techniques has the potential to provide insights into host-virus interactions at the single-cell level.
FAQs
The signal arises from an interaction between the assay component and the RNA target. A fluorescently labeled probe can bind a specific sequence, while a dye or RNA-binding reporter can respond to RNA association or a structural change. That interaction alters fluorescence, allowing the instrument to connect the measured signal with the presence or amount of the target RNA.
Target-sequence recognition helps the assay focus on a particular transcript rather than treating all RNA as equivalent. A probe that interacts with its intended sequence can support detection of specific transcripts, while the resulting fluorescence provides a measurable readout. This makes the approach useful for examining gene-expression patterns and distinguishing one RNA target from another.
Fluorescence can provide information about where RNA is located and how its signal changes within a biological system, not only how much RNA is present. When paired with microscopy or another suitable analytical platform, the assay can support studies of RNA localization and dynamics. These measurements add spatial or time-related context to molecular detection.
The measured fluorescence serves as an indirect readout of the RNA-related interaction occurring in the assay. Depending on the design, the signal can be used to estimate RNA abundance, indicate detection of a specific transcript, or reflect a binding or structural change. Interpretation therefore depends on what the probe, dye, or reporter was designed to monitor.
A typical workflow establishes contact between the RNA-containing sample and a fluorescently labeled probe, dye, or RNA-binding reporter. The resulting fluorescence is then measured with an appropriate instrument, and the signal is interpreted to estimate RNA or identify a target transcript. The same general workflow can be adapted for abundance measurements, purification assessment, or biological tracking.
A microplate reader is suited to fluorescence measurements across assay samples, supporting rapid analysis and comparison of RNA-related signals. Microscopy is more appropriate when the goal includes observing RNA localization or dynamics in biological systems. Thus, the platform reflects the information sought: broader quantitative measurement for plate-based analysis or visual biological context for imaging.
Fluorescence provides a measurable signal that can help researchers examine RNA-containing preparations during purification and quality assessment. By tracking the RNA-related signal, they can obtain information about whether RNA is present and evaluate samples using an analytical readout rather than relying only on visual inspection. This supports laboratory workflows in which RNA preparation precedes downstream biological analysis.
These assays support gene-expression analysis, detection of specific transcripts, RNA purification and quality assessment, and investigations of RNA localization or dynamics. Their compatibility with microplate readers, microscopy, and other analytical platforms broadens how results can be collected. The same flexibility also makes them relevant to diagnostic development, where sensitive RNA detection and measurement are important goals.