Human Endometrial Xenograft
Human Endometrial Xenograft - Related Videos
09:15
Two Methods for Establishing Primary Human Endometrial Stromal Cells from Hysterectomy Specimens
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Cited by 14 •
2014
Establishing primary endometrial stromal cell culture systems from hysterectomy specimens is a valuable biological technique and a crucial step prior to pursuing a vast array of research aims. Here, we describe two methods used to establish stromal cultures from surgically resected endometrial tissues of human...
02:26
A Technique to Generate a Human PBMC-Engrafted Humanized Xenograft Mouse Model
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2025
This video demonstrates an assay to generate a humanized xenograft mouse model. An immunocompromised mouse is injected with cyclophosphamide to cause immune cell depletion. Patient-derived tumor cells mixed with human peripheral blood mononuclear cells (PBMCs) in a hydrogel are subcutaneously injected into the mouse, forming a tumor with infiltrating T cells.
11:44
Isolation of Stem Cells from Human Pancreatic Cancer Xenografts
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Cited by 40 •
2010
Cancer stem cells (CSCs) have been identified in a number of malignancies. In this protocol we describe a flow cytometric method utilizing aldehyde dehydrogenase activity and CD44 and CD24 expression to isolate CSCs from human pancreatic adenocarcinoma xenografts. These viable cells can then be used in functional and analytical studies.
10:35
Generation of Subcutaneous and Intrahepatic Human Hepatocellular Carcinoma Xenografts in Immunodeficient Mice
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Cited by 15 •
2013
Human tumor xenografts in immunodeficient mice are valuable tools to study cancer biology. Specific protocols to generate subcutaneous and intrahepatic xenografts from human hepatocellular carcinoma cells or tumor fragments are described. Liver regeneration induced by partial hepatectomy in recipient mice is presented as a strategy to facilitate intrahepatic engraftment.
10:52
Tumor Engraftment in a Xenograft Mouse Model of Human Mantle Cell Lymphoma
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Cited by 1 •
2018
Mantle cell lymphoma (MCL) is a difficult to treat B cell disorder and it is equally difficult to establish a xenograft mouse model of primary MCL to study and develop therapeutics. Here, we describe the successful establishment of MCL xenografts in mice to help understand its underlying biology.
FAQs
The reduced immune response is central because it permits transplanted human endometrial cells to remain in the animal and establish tissue behavior that can be observed in vivo. This permissive host environment makes it possible to examine how the graft responds to surrounding tissues and biological signals rather than being eliminated immediately.
Hormonal signals help drive the graft’s cyclical behavior. By retaining responsiveness to these signals, transplanted tissue can be examined as it changes over time, including hormone-dependent growth. This feature allows investigators to connect endocrine regulation with tissue responses relevant to endometrial biology and disease-related growth patterns within a living host.
Human endometrial xenografts are useful for studying endometriosis because they can support analysis of several linked processes in the same experimental setting. Investigators can examine lesion establishment alongside inflammation and vascularization, helping relate visible tissue development to biological mechanisms that contribute to disease progression in vivo.
Compared with cell cultures, this model preserves tissue-level interactions within a living organism. Those interactions may reveal responses that isolated cells do not reproduce, including communication between transplanted endometrial tissue and its surrounding environment. Consequently, the xenograft provides a biological context for interpreting growth, inflammatory changes, and vascular development.
A basic study begins by transplanting human endometrial tissue into an immunocompromised animal, then observing how the graft behaves in vivo. Researchers can assess cyclical changes, hormone-dependent growth, lesion formation, inflammation, and vascularization. The sequence links tissue implantation with measurable biological outcomes while maintaining the living-host context.
Researchers can use the xenograft to investigate how endometrial tissue behaves under disease-relevant conditions, especially when studying endometriosis. The model supports examination of lesion establishment, inflammatory activity, vascularization, and hormone-dependent growth together. This combination helps connect cellular behavior with changes occurring at the tissue level in a living organism.
Human endometrial xenograft models also serve as a preclinical platform for assessing potential therapeutic strategies. Because the graft retains hormone-responsive and tissue-level behavior, investigators can observe whether an intervention changes disease-associated growth or related biological features before clinical studies. The model therefore connects mechanistic biology with early evaluation of treatment approaches.