Syringe Preparation
Syringe Preparation - Related Videos
02:28
Extraction of Apoplastic Bacteria Using a Syringe-Based Pressure Method
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2025
Source: Rufián, J. S., et al, Single-Cell Analysis of the Expression of Pseudomonas syringae Genes within the Plant Tissue. J. Vis. Exp. (2022)This video demonstrates a syringe-based pressure method for extracting apoplastic bacteria, which proliferate and form microcolonies within the apoplast, the intercellular space between plant cells. The method yields debris-free bacterial samples suitable for downstream single-cell analysis.
09:58
Syringe-injectable Mesh Electronics for Stable Chronic Rodent Electrophysiology
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Cited by 31 •
2018
Mesh electronics probes seamlessly integrate and provide stable, long-term, single-neuron level recording within the brain. This protocol uses mesh electronics for in vivo experiments, involving the fabrication of mesh electronics, loading into needles, stereotaxic injection, input/output interfacing, recording experiments, and histology of tissue containing mesh probes.
05:49
Modeling the Effects of Hemodynamic Stress on Circulating Tumor Cells using a Syringe and Needle
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Cited by 2 •
2021
Here we demonstrate a method to apply fluid shear stress to cancer cells in suspension to model the effects of hemodynamic stress on circulating tumor cells.
09:08
Three-dimensional Printing of Thermoplastic Materials to Create Automated Syringe Pumps with Feedback Control for Microfluidic Applications
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Cited by 3 •
2018
Here we present a protocol to construct a pressure-controlled syringe pump to be used in microfluidic applications. This syringe pump is made from an additively manufactured body, off-the-shelf hardware, and open-source electronics. The resulting system is low-cost, straightforward to build, and delivers well-regulated fluid flow to enable rapid microfluidic research.
09:51
Sample Preparation for Analytical Characterization
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2023
Source: Laboratory of Dr. B. Jill Venton - University of Virginia Sample preparation is the way in which a sample is treated to prepare for analysis. Careful sample preparation is critical in analytical chemistry to accurately generate either a standard or unknown sample for a chemical measurement. Errors in analytical chemistry methods are categorized as random or systematic. Random errors are errors due to change and are often due to noise in instrument. Systematic errors are due to...
FAQs
Air bubbles can compromise the volume actually measured or delivered. In biological work, that uncertainty matters when transferring samples, handling reagents, or dosing materials. Removing bubbles supports reliable volume control and helps experiments produce reproducible results, particularly when consistent transfer is important for cell cultures, biological fluids, or other sensitive specimens.
The syringe and needle must be selected as compatible components before assembly. Compatibility supports secure attachment and helps the system function as intended during withdrawal and delivery. This consideration is important when handling biological samples or reagents because an unsuitable or insecure connection can undermine measurement reliability and increase the risk of contamination.
Inspection provides an initial check that the components are appropriate for the planned biological task and can be assembled securely. This step supports safe handling before liquid is withdrawn or delivered. It also helps protect samples, reagents, specimens, and users by addressing component suitability before the transfer process begins.
A consistent sequence begins with inspecting the syringe and needle, selecting compatible components, and attaching the needle securely. The required liquid volume is then drawn, followed by removal of visible air bubbles. Following these stages in the same order supports accurate measurement, preserves sterility, and promotes reproducible handling across biological experiments.
The procedure is useful whenever biological work requires controlled liquid transfer or dosing. Applications described for this setting include sample transfer, reagent handling, injections, and dosing, as well as work involving cell cultures and biological fluids. Its value is greatest when accurate delivery and protection against contamination influence the experiment.
Careful preparation supports accurate measurement, protects users and specimens, and helps prevent contamination. These effects are especially relevant when researchers handle sensitive biological materials or perform repeated transfers. By promoting consistent volumes and preserving sterility, the process contributes to more reliable and reproducible outcomes in laboratory and research settings.