Microvasculature Assessment
Microvasculature Assessment - Related Videos
04:11
DCE-MRI of Orthotopic Pancreatic Tumor Xenografts: A Method to Assess Microvasculature in a Target Tumor Tissue
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2023
This video describes the technique of DCE-MRI for orthotropic pancreatic tumor xenografts in mice. DCE-MRI is a non-invasive method to analyze microvasculature in a target tissue, and useful to assess vascular response in a tumor following a novel therapy. This method will assist investigators to apply DCE-MRI for orthotopic gastrointestinal cancer mouse models.
02:58
Two-Photon Microscopy for In Vivo Imaging of Mouse Brain Microvasculature
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2025
Source: Nishimura, C. et al. Quantification of Cerebral Vascular Architecture using Two-photon Microscopy in a Mouse Model of HIV-induced Neuroinflammation. J. Vis. Exp. (2016). This video demonstrates the in vivo visualization of cortical capillaries in the mouse brain using two-photon microscopy. It outlines the steps for injecting a fluorescent dye into an anesthetized mouse with a thin-skull cortical window, preparing the setup for imaging, and acquiring detailed images of brain...
09:39
Spatial Temporal Analysis of Fieldwise Flow in Microvasculature
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Cited by 7 •
2019
To quantify microvascular flow from high speed capillary flow image sequences, we developed STAFF (Spatial Temporal Analysis of Fieldwise Flow) software. Across the full image field and over time, STAFF evaluates flow velocities and generates a sequence of color-coded spatial maps for visualization and tabular output for quantitative analyses.
11:40
Humanized Mouse Model to Study Bacterial Infections Targeting the Microvasculature
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Cited by 7 •
2014
Neisseria meningitidis is a human specific pathogen that infects blood vessels. In this protocol human microvessels are introduced into a mouse by grafting human skin onto immunocompromised mice. Bacteria adhere extensively to the human vessels, leading to vascular damage and development of the purpuric rash typically observed in human cases.
10:36
Ultrasound Localization Microscopy for Super-Resolution Mapping of the Rodent Brain Microvasculature
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2025
Ultrasound Localization Microscopy (ULM) is a recently developed technique that allows for an unprecedented level of resolution in imaging the microvasculature in vivo. In this work, we describe in detail how to obtain super-resolved images of the brain microvasculature in rodents using a standardized functional ultrasound imaging platform designed for preclinical research.
FAQs
Angiogenic signaling can drive the formation of irregular tumor-associated vessels rather than a stable vascular pattern. These structural changes may influence how blood moves through the tumor and how readily substances pass across vessel walls. Assessing vessel organization together with perfusion and permeability helps researchers connect signaling activity with tumor growth, invasion, and treatment resistance.
A vessel count alone may not capture how effectively a tumor is supplied or how substances move between blood and tissue. Combining density with blood flow and permeability measurements provides a broader view of vascular function. This integrated assessment can clarify whether vascular changes are associated with altered perfusion, the tumor microenvironment, or potential differences in treatment response.
Longitudinal changes in small-vessel structure can indicate that the tumor microenvironment is being remodeled as disease develops. Comparing measurements across progression can reveal shifts in vessel density, perfusion, or permeability rather than relying on a single endpoint. Such patterns help researchers examine how vascular alterations may accompany tumor growth and invasion.
Repeated or comparative measurements establish how a tumor’s vascular features change after therapy relative to its earlier state. Researchers can examine structural, flow-related, or permeability changes and relate them to treatment exposure. This approach supports evaluation of antiangiogenic and other cancer treatments, while helping distinguish vascular response patterns from characteristics present before intervention.
A study generally identifies the vascular features relevant to the research question, selects imaging or quantitative measurements, and evaluates vessel structure, density, blood flow, or permeability. Researchers then compare results across tumors, disease stages, or treatment conditions. The selected measurements should match the intended outcome, such as monitoring progression, treatment response, or delivery-related vascular changes.
Vascular measurements can help researchers investigate how tumor-associated vessels affect the movement of treatments through the tumor microenvironment. Assessing blood flow and permeability provides context for differences in delivery, while structural measurements describe the vascular environment in which delivery occurs. These data can support studies of drug delivery and interpretation of variable treatment outcomes.
Quantitative vascular features can be compared with disease progression or response to therapy to identify measurements associated with biologically meaningful changes. Structure, density, flow, and permeability each provide a different type of information about the tumor-associated vascular environment. Together, they may support evaluation of candidate biomarkers for tumor biology, treatment monitoring, or therapeutic response.