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Spatial Temporal Analysis of Fieldwise Flow in Microvasculature
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Bioengineering
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JoVE Journal Bioengineering
Spatial Temporal Analysis of Fieldwise Flow in Microvasculature

Spatial Temporal Analysis of Fieldwise Flow in Microvasculature

DOI:
10.3791/60493-v

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09:39 min

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November 18, 2019

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, , , , , , , , , , ,
1Biocomplexity Institute,Indiana University, 2Department of Intelligent Systems Engineering,Indiana University, 3Department of Physics,Indiana University, 4Scientific Designs, 5Department of Medicine,Indiana University, 6School of Public Health,Indiana University

Chapters

  • 00:04Title
  • 00:39Vascular Network Definition using TrakEM2 in FIJI
  • 03:52Vascular Flow Quantification Using STAFF
  • 07:26Results: Representative Vascular Skeleton Generation and Kymograph and Velocity Data Analyses
  • 08:58Conclusion

Summary

Automatic Translation

Automatic Translation

To quantify microvascular flow from high speed capillary flow image sequences, we developed STAFF (Spatial Temporal Analysis of Fieldwise Flow) software. Across the full image field and over time, STAFF evaluates flow velocities and generates a sequence of color-coded spatial maps for visualization and tabular output for quantitative analyses.

Tags

Keywords: Spatial Temporal AnalysisMicrovascular FlowFlow VelocitiesTrakEM2Vascular NetworkArea ListPaintbrush ToolImage ProcessingFijiBrightness/contrast

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