Stool Collection

Stool collection is the standardized process of obtaining and preserving fecal material for laboratory analysis, providing a noninvasive source of biological information relevant to cancer research. Reliable collection requires a clean, contamination-free container, consistent handling, and appropriate storage to help maintain microbial, cellular, genetic, and chemical components in the sample. Researchers use collected stool to characterize gut microbiome composition, measure metabolites, and investigate potential biomarkers associated with cancer risk, treatment response, or disease progression. Careful standardization improves sample comparability across participants and strengthens studies of interactions between intestinal biology, tumor development, and therapeutic outcomes.

Stool Collection - Related Videos

Research

JoVE Journal - Medicine

Detection and Genogrouping of Noroviruses from Children's Stools By Taqman One-step RT-PCR

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Cited by 13 •

2012

A One-Step RT-PCR assay for detection and genogroup identification of Norovirus isolates from children’s stools, that utilizes primers and TaqMan probes specific to the open reading frame 1 (ORF1)-ORF2 junction region, the most conserved region of the Norovirus genome is described. A non-commercial, cost-effective RNA extraction method is detailed.

Stool Microbial DNA Isolation: Studying the Effect of Environmental Enrichment on Microbiome Diversity in Murine Tumor Model

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2023

This video describes DNA isolation protocol from stool samples of colon tumor-bearing mice. The extracted DNA is amplified using PCR, and the product is further cleaned using a magnetic beads-based approach. This method can be used to determine the effect of environmental enrichment on colon microbiota and animal mortality.

Education

JoVE Core - Medical-Surgical Nursing

Serum Laboratory Studies, Stool Test, Breath Test

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2025

Gastrointestinal (GI) diagnostic studies are pivotal in confirming, ruling out, diagnosing, or staging various diseases, including cancers. Following diagnosis, allocating time for discussions with the patient and providing informational resources is crucial. Diagnostic assessments of the GI tract often occur in outpatient settings like endoscopy suites or GI labs. Preparation for these tests may include dietary restrictions, fasting, liquid bowel preparations, laxatives, enemas, and the...

Seawater Sampling and Collection

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Cited by 13 •

2009

This video documents methods for collecting coastal marine water samples and processing them for various downstream applications including biomass concentration, nucleic acid purification, cell abundance, nutrient and trace gas analyses.

Data Collection II

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2023

The nursing history captures and records the patient's health status, so that a care plan evolves to meet the patient's individual needs. The nursing health history is a part of the initial assessment. A comprehensive history covers all health dimensions and plays a significant role in the assessment process. A comprehensive history includes the patient's biographical information, reasons for seeking health care, expectations, present and past health history, medications, and family,...

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