Tumor Progression Metastasis Study
Tumor Progression Metastasis Study - Related Videos
06:31
Primary Tumor and MEF Cell Isolation to Study Lung Metastasis
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Cited by 13 •
2015
The goal of this protocol is to study breast tumorigenesis. With this technique, mouse mammary tumors are removed and primary cells are prepared from tumors. A lung extraction protocol is included for studying lung metastasis. Furthermore, another protocol for analyzing mouse embryonic fibroblasts from the mouse embryo is included.
12:03
In Vivo Model for Testing Effect of Hypoxia on Tumor Metastasis
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Cited by 6 •
2016
This manuscript describes the development of an animal model that allows for the direct testing of the effects of tumor hypoxia on metastasis and the deciphering the mechanisms of its action. Although the experiments described here focus on Ewing sarcoma, a similar approach can be applied to other tumor types.
09:53
Labeling of Breast Cancer Patient-derived Xenografts with Traceable Reporters for Tumor Growth and Metastasis Studies
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Cited by 18 •
2016
We describe a method for stable labeling of patient-derived xenografts (PDXs) with lentiviral particles expressing green-fluorescent protein and luciferase reporters. This method allows for tracking the growth of PDXs at the primary site, as well as detecting spontaneous and experimental metastases using in vivo imaging systems.
03:46
Bioluminescent Imaging of Breast Tumor Progression in a Female Mouse Model
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2025
Source: Marina Arantes Radicchi1, Victor Carlos Mello1, Victória Paz Machado1, Márcia Cristina Oliveira da Rocha1, Jaqueline Vaz de Oliveira1, Luana Cristina Camargo1,2, Luís Alexandre Muehlmann1, Ricardo Bentes Azevedo1, Sônia Nair Bao1, João Paulo Figueiró Longo11Institute of Biological Sciences, University of Brasília, 2Department of Basic Psychological Processes, Institute of Psychology, University of BrasíliaThis video demonstrates the procedure for tracking tumor progression in vivo by...
02:07
Tumor Progression
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2023
Tumor progression is a phenomenon where the pre-formed tumor acquires successive mutations to become clinically more aggressive and malignant. In the 1950s, Foulds first described the stepwise progression of cancer cells through successive stages. Colon cancer is one of the best-documented examples of tumor progression. Early mutation in the APC gene in colon cells causes a small growth on the colon wall called a polyp. With time, this polyp grows into a benign, pre-cancerous tumor. Further...
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Genetic and epigenetic changes can increase cancer-cell proliferation, modify interactions with the tumor microenvironment, and promote traits associated with invasion. These alterations help explain why cells within a primary tumor may behave differently and why some tumors advance more rapidly. Studying them can reveal mechanisms linked to disease progression and potential biomarkers of aggressive disease.
Successful dissemination requires several sequential events: invasion from the primary tumor, intravasation into blood or lymphatic vessels, survival during circulation, extravasation into another tissue, and colonization. Failure at any stage can limit metastatic spread. Examining these transitions helps investigators identify which steps most strongly influence dissemination and where therapeutic intervention might be effective.
Tumor progression and metastasis studies investigate organ preference by following how cancer cells interact with distant tissues after dissemination. The ability to extravasate and colonize is not equivalent across organs, so examining these stages can clarify why secondary disease develops in particular locations. This information may improve interpretation of metastatic patterns and support organ-specific research questions.
The tumor microenvironment can influence how cancer cells proliferate, interact with surrounding tissues, and acquire invasive behavior. Because progression is shaped by both cancer-cell changes and these local interactions, studies do not focus only on tumor cells in isolation. Evaluating the surrounding environment can reveal mechanisms that contribute to advancement and identify additional targets for treatment development.
Investigators can examine whether a therapy affects tumor growth, aggressive traits, or distinct stages of dissemination. Relevant outcomes include changes in primary-tumor expansion, invasion, intravasation, circulation survival, extravasation, or colonization. Comparing these effects helps distinguish treatments that mainly limit local progression from those that may also reduce metastatic spread.
By linking cellular changes and metastatic behavior with disease advancement, these studies can support the identification of biomarkers associated with progression. Such markers may help researchers improve earlier detection and prognosis, while stage-specific findings can guide treatment development. The broader goal is to translate mechanistic observations into strategies for preventing or treating advanced cancer.