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Labeling of Breast Cancer Patient-derived Xenografts with Traceable Reporters for Tumor Growth and Metastasis Studies
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Cancer Research
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JoVE Journal Cancer Research
Labeling of Breast Cancer Patient-derived Xenografts with Traceable Reporters for Tumor Growth and Metastasis Studies

Labeling of Breast Cancer Patient-derived Xenografts with Traceable Reporters for Tumor Growth and Metastasis Studies

DOI:
10.3791/54944-v

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09:53 min

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November 30, 2016

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, , , ,
1Department of Pathology, School of Medicine,University of Colorado Anschutz Medical Campus

Chapters

  • 00:05Title
  • 00:58Dissection and Dissociation of Patient-derived Xenograft (PDX)-derived Tumor Cells
  • 03:20Human Epithelial Cancer Cell Enrichment
  • 05:13PDX-derived Tumor Cell Transduction, Transduction Efficiency Evaluation and Tumor Cell Re-implantation
  • 06:48Results: PDX-dissociated Breast Cancer Cell Transduction Using High Titer Lentiviral Vectors
  • 08:33Conclusion

Summary

Automatic Translation

Automatic Translation

We describe a method for stable labeling of patient-derived xenografts (PDXs) with lentiviral particles expressing green-fluorescent protein and luciferase reporters. This method allows for tracking the growth of PDXs at the primary site, as well as detecting spontaneous and experimental metastases using in vivo imaging systems.

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Keywords: Breast CancerPatient-derived XenograftsPDXReporter GenesTumor GrowthMetastasisIn Vivo ImagingAseptic TechniqueCell IsolationRed Blood Cell Lysis

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