Nanoprobe Detection Method
Nanoprobe Detection Method - Related Videos
02:59
A Technique to Detect Extrapulmonary Tuberculosis via Superparamagnetic Nanoprobes
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2025
This video demonstrates a technique for the detection of extrapulmonary tuberculosis. Superparamagnetic iron oxide (SPIO) nanoprobes, conjugated to Mycobacterium-specific antibodies, are injected into a mouse previously infected with Mycobacterium bovis BCG, labeling the granuloma and providing a better imaging contrast for its detection.
03:17
Antibody-Functionalized SERRS Nanoprobe Based Imaging: A Highly Sensitive Raman-imaging Technique to Detect Metastatic Ovarian Cancer In Vivo
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2023
This video describes the surface-enhanced resonance Raman scattering technique to detect metastatic ovarian cancer using targeted nanoprobes specific anti-receptor antibodies and non-targeted control nanoprobes. This method helps to determine the relative abundance of the two nanoprobes and differentiate the cancer cells from the visceral background.
09:54
Synthesis, Characterization, and Application of Superparamagnetic Iron Oxide Nanoprobes for Extrapulmonary Tuberculosis Detection
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Cited by 1 •
2020
To improve serological diagnostic tests for Mycobacterium tuberculosis antigens, we developed superparamagnetic iron oxide nanoprobes to detect extrapulmonary tuberculosis.
10:05
High-throughput Detection Method for Influenza Virus
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Cited by 8 •
2012
This method describes the use of Infrared dye based imaging system for detection of H1N1 in bronchioalveolar lavage (BAL) fluid of infected mice at a high sensitivity. This methodology can be performed in a 96- or 384-well plate, requires <10 μl volume of test material and has the potential for concurrent screening of multiple pathogens.
07:54
Surface-enhanced Resonance Raman Scattering Nanoprobe Ratiometry for Detecting Microscopic Ovarian Cancer via Folate Receptor Targeting
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Cited by 17 •
2019
Ovarian cancer forms metastases throughout the peritoneal cavity. Here, we present a protocol to make and use folate-receptor targeted surface-enhanced resonance Raman scattering nanoprobes that reveal these lesions with high specificity via ratiometric imaging. The nanoprobes are administered intraperitoneally to living mice, and the derived images correlate well with histology.
FAQs
The process links analyte recognition to a change in an observable signal. When the target interacts with the nanoprobe, that interaction may alter fluorescence, light scattering, color, or an electrochemical response. Measuring the resulting change allows the chemical or biological target to be detected, including substances present at very low concentrations.
Nanoscale surface properties help the probe interact effectively with the target and can contribute to signal amplification. This combination strengthens the observable response produced by analyte recognition, improving detection limits. In chemistry, that advantage supports analysis when the target is present at concentrations too low for less sensitive approaches.
Selectivity depends on how specifically the nanoprobe interacts with the intended analyte in a complex sample. The relevant target may be an ion, molecule, pollutant, or reaction product, while other sample components can also be present. Designing the recognition interaction and monitoring its associated signal are therefore central to reliable identification.
These signal types provide different observable readouts of the nanoprobe-analyte interaction. A change in fluorescence, light scattering, color, or electrochemical response indicates that the probe has responded to a target. The selected readout determines how the interaction is observed and can support rapid chemical analysis across varied sample types.
A general workflow introduces the nanoprobe to a sample containing a possible target, allows the relevant interaction to occur, and observes the resulting signal change. The response is then measured to determine whether the analyte is present and to support analysis at low concentration. The precise readout depends on the probe and target.
This approach is useful when analysis must detect ions, molecules, pollutants, or other chemical and biological targets at very low concentrations. Environmental monitoring can use it to examine pollutants, while biomedical testing can apply the same sensing principle to relevant targets in complex samples. Its sensitivity and rapid analysis support both settings.
Nanoprobe detection can contribute to portable analytical devices because the method produces measurable optical or electrochemical responses without requiring the target to be present at high concentration. Portable formats may support rapid analysis outside conventional laboratory settings, extending chemical sensing to environmental monitoring, biomedical testing, and other situations requiring accessible measurements.