Surface-enhanced Resonance Raman Scattering Nanoprobe Ratiometry for Detecting Microscopic Ovarian Cancer via Folate Receptor Targeting

Chrysafis Andreou; Anton Oseledchyk; Fay Nicolson; Naxhije Berisha; Suchetan Pal; Moritz F. Kircher

doi:10.3791/58389

/

Surface-enhanced Resonance Raman Scattering Nanoprobe Ratiometry for Detecting Microscopic Ovarian Cancer via Folate Receptor Targeting

JoVE Journal
Cancer Research

A subscription to JoVE is required to view this content. Sign in or start your free trial.

JoVE Journal Cancer Research

Surface-enhanced Resonance Raman Scattering Nanoprobe Ratiometry for Detecting Microscopic Ovarian Cancer via Folate Receptor Targeting

8,118 Views

•

07:54 min

•

March 25, 2019

DOI:

10.3791/58389-v

DOI:

10.3791/58389-v

•

07:54 min

March 25, 2019

•

8106 Views

Chrysafis Andreou¹, Anton Oseledchyk¹, Fay Nicolson¹, Naxhije Berisha^1,2, Suchetan Pal¹, Moritz F. Kircher^1,3,4,5,6,7

¹Department of Radiology,Memorial Sloan Kettering Cancer Center ²Department of Chemistry,The Graduate Center of the City University of New York ³Molecular Pharmacology Program,Memorial Sloan Kettering Cancer Center ⁴Center for Molecular Imaging and Nanotechnology (CMINT),Memorial Sloan Kettering Cancer Center ⁵Gerstner Sloan Kettering Graduate School of Biomedical Sciences ⁶Department of Radiology,Weill Cornell Medical College of Cornell University ⁷Dana-Farber Cancer Institute and Harvard Medical Center

Summary

Ovarian cancer forms metastases throughout the peritoneal cavity. Here, we present a protocol to make and use folate-receptor targeted surface-enhanced resonance Raman scattering nanoprobes that reveal these lesions with high specificity via ratiometric imaging. The nanoprobes are administered intraperitoneally to living mice, and the derived images correlate well with histology.