Fluid Aspiration
Fluid Aspiration - Related Videos
06:43
Fluid Aspiration from Mouse Glomeruli: A Method to Aspirate Glomerular Fluid from Mouse Using Two-Photon Mediated Micropuncture
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2023
This video showcases fluid aspiration from mouse glomeruli using the two-photon mediated micropuncture technique. The technique provides access to the living mouse’s glomeruli and thus helps gain insights into renal physiology.
06:41
A Technique for Percutaneous Epididymal Spermatozoa Aspiration and Processing
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2026
Source:Li Xiaoming1, Liu Xiao1, Li Haiyang1, Cai Xiaohua1, Ou Jianping11Center for Reproductive Medicine, The Third Affiliated Hospital of Sun Yat-sen University.This video demonstrates the percutaneous aspiration of epididymal spermatozoa from a patient with azoospermia, a condition characterized by the absence of spermatozoa in the semen. The procedure involves disinfecting the scrotal area, aspirating epididymal fluid using a syringe with a specialized culture solution and processing the...
07:10
Femoral Bone Marrow Aspiration in Live Mice
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Cited by 18 •
2014
This protocol describes a procedure for serial sampling of femoral bone marrow (BM) without requiring the sacrifice of mice. This procedure facilitates longitudinal studies of the BM composition of mice over time and provides serial access to cells within the BM for ex vivo and transplantation studies.
02:43
Fly Aspirator Tube: A Simple Tool to Transfer Live Drosophila
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2023
This video describes one method to build a fly aspirator, which can be used to transfer insects between enclosures without anesthesia. The aspirator in the example protocol was prepared for an experiment to quantify individual grooming...
10:31
Micropipette Aspiration of Substrate-attached Cells to Estimate Cell Stiffness
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Cited by 30 •
2012
Here we describe a quick and simple method to measure cell stiffness. The general principle of this approach is to measure membrane deformation in response to well-defined negative pressure applied through a micropipette to the cell surface. This method provides a powerful tool to study biomechanical properties of substrate-attached cells.
FAQs
Negative pressure creates a pressure difference between the collection and the device, allowing liquid to move into a needle, catheter, or similar instrument. This controlled suction is central to obtaining material from a body cavity, tissue, or fluid collection. The resulting sample can support diagnosis, while removal of volume can decompress the space and relieve pressure-related symptoms.
Targeting accuracy depends on identifying the fluid-filled space and avoiding nearby structures. Ultrasound or other imaging may guide device placement when needed. Imaging therefore contributes not only to successful fluid recovery but also to safer drainage by helping clinicians select an appropriate path into the collection.
Analysis may assess the material for signs of infection, blood, malignant cells, or other abnormalities. These findings give the procedure a diagnostic role beyond simply removing liquid. The result can help clinicians characterize the collection and guide further management, although interpretation depends on the clinical context and the purpose for which the sample was obtained.
Clinicians identify the target collection, select a needle, catheter, or similar device, and use it to create negative pressure and collect or remove fluid. Imaging may assist targeting. Afterward, the material can be examined diagnostically, or drainage can be used therapeutically when decompression is the goal.
Therapeutic aspiration is used when removing liquid can decompress a fluid-filled space or relieve pressure and associated symptoms. In this setting, the primary outcome is not only information from the material but also reduction of the collection’s volume. Diagnostic sampling and therapeutic drainage may occur together when both questions matter.
Proper technique and accurate targeting are central because the device may pass close to nearby structures. Careful performance helps limit bleeding, infection, and injury to surrounding anatomy. The balance between obtaining or removing enough fluid and maintaining a safe approach is therefore important in both diagnostic sampling and therapeutic drainage.