Human Cardiac Tissue
Human Cardiac Tissue - Related Videos
11:51
Construction of Defined Human Engineered Cardiac Tissues to Study Mechanisms of Cardiac Cell Therapy
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Cited by 16 •
2016
This manuscript describes the creation of defined engineered cardiac tissues using surface marker expression and cell sorting. The defined tissues can then be used in a multi-tissue bioreactor to investigate mechanisms of cardiac cell therapy in order to provide a functional, yet controlled, model system of the human heart.
08:15
Isolation of Perivascular Multipotent Precursor Cell Populations from Human Cardiac Tissue
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Cited by 7 •
2016
Human cardiac tissue harbours multipotent perivascular precursor cell populations that may be suitable for myocardial regeneration. The technique described here allows for the simultaneous isolation and purification of two multipotent stromal cell populations associated with native blood vessels, i.e. CD146+CD34- pericytes and CD34+CD146- adventitial cells, from the human myocardium.
04:40
Creation of Cardiac Tissue Exhibiting Mechanical Integration of Spheroids Using 3D Bioprinting
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Cited by 55 •
2017
This protocol describes 3D bioprinting of cardiac tissue without the use of biomaterials. 3D bioprinted cardiac patches exhibit mechanical integration of component spheroids and are highly promising in cardiac tissue regeneration and as 3D models of heart disease.
10:09
Capillary Force Lithography for Cardiac Tissue Engineering
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Cited by 27 •
2014
In this protocol, we demonstrate the fabrication of biomimetic cardiac cell culture substrata made from two distinct polymeric materials using capillary force lithography. The described methods provide a scalable, cost-effective technique to engineer the structure and function of macroscopic cardiac tissues for in vitro and in vivo applications.
10:39
Automated Contraction Analysis of Human Engineered Heart Tissue for Cardiac Drug Safety Screening
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Cited by 38 •
2017
Here, we show the generation of human engineered heart tissue from induced pluripotent stem cells (hiPSC)-derived cardiomyocytes. We present a method to analyze contraction force and exemplary alteration of contraction pattern by the hERG channel inhibitor E-4031. This method shows high level of robustness and suitability for cardiac drug screening.
FAQs
Cardiomyocytes generate force through interactions between actin and myosin, the contractile proteins within these cells. Electrical signals then spread through specialized connections between cells, coordinating their activity rather than allowing contractions to occur independently. This coupling is essential for organized cardiac performance and provides a basis for studying how structural and electrical properties work together.
Cardiac tissue contains more than contractile cardiomyocytes. Connective tissue and vascular components form part of the tissue context in which cardiac cells develop and function. Including these components allows investigations to consider tissue organization alongside force production and electrical coordination, helping researchers examine cardiac structure as an integrated biological system rather than as isolated muscle cells.
During development, progenitor cells differentiate into several cardiac cell types and then participate in the organization of the forming heart. Their regulated progression contributes to the emergence of chambers, valves, and conduction structures. Studying this process helps developmental biologists connect early cell-fate decisions with the later anatomical organization required for cardiac function.
Cardiac development depends on tightly regulated molecular and mechanical cues that influence both differentiation and tissue organization. These signals help guide progenitor cells toward cardiac cell types and support the formation of distinct structures, including chambers, valves, and conduction regions. Examining both cue categories is important because developmental outcomes reflect coordinated changes in cell identity and tissue architecture.
Human cardiac tissue provides a context for examining how abnormal development may affect cardiac organization. Researchers can relate progenitor-cell differentiation and the formation of chambers, valves, or conduction structures to congenital defects. This developmental perspective helps identify which stages or organizational processes may be disrupted, making the tissue relevant to models of heart formation and disease.
Studies of human cardiac tissue support disease modeling by providing a system in which cardiac cell types, tissue organization, force generation, and electrical coordination can be examined together. The same features make it relevant for drug testing, where researchers can investigate how candidate treatments affect cardiac tissue properties and developmental or disease-related phenotypes.
Human cardiac tissue research helps examine how cardiac tissue responds to injury and how its organized cellular components might be restored. Because the tissue includes cardiomyocytes, connective tissue, vascular components, and conduction structures, regenerative studies can consider more than replacement of contractile cells alone. This supports broader efforts to understand tissue repair and cardiac recovery.