Murine Auditory Organs
Murine Auditory Organs - Related Videos
06:45
Gene Transfer into the Chicken Auditory Organ by In Ovo Micro-electroporation
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Cited by 3 •
2016
The auditory organ mediates hearing. Here we present a modified in ovo micro-electroporation method optimized for studying auditory progenitor cell proliferation and differentiation in the developing chicken auditory organ.
07:58
Direct Visualization of the Murine Dorsal Cochlear Nucleus for Optogenetic Stimulation of the Auditory Pathway
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Cited by 10 •
2015
The goal of this protocol is to outline a surgical approach to provide direct access to the dorsal cochlear nucleus in a murine model.
08:17
Three-dimensional Organotypic Cultures of Vestibular and Auditory Sensory Organs
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Cited by 5 •
2018
Three-dimensional organotypic cultures of the murine utricle and cochlea in optically clear collagen I gels preserve innate tissue morphology, allow for mechanical stimulation through adjustment of matrix stiffness, and permit virus-mediated gene delivery.
09:35
Automatic Detection of Highly Organized Theta Oscillations in the Murine EEG
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Cited by 5 •
2017
Theta activity in the hippocampus is related to specific cognitive and behavioral stages. Here, we describe an analytical method to detect highly-organized theta oscillations within the hippocampus using a time-frequency (i.e., wavelet analysis)-based approach.
10:14
In Ovo Electroporation in the Chicken Auditory Brainstem
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Cited by 6 •
2017
Auditory brainstem neurons of avians and mammals are specialized for fast neural encoding, a fundamental process for normal hearing functions. These neurons arise from distinct precursors of embryonic hindbrain. We present techniques utilizing electroporation to express genes in the hindbrain of chicken embryos to study gene function during auditory development.
FAQs
Development begins when the otic placode forms and then produces the otic vesicle. From that vesicle, patterned growth and differentiation progressively establish the cochlea, vestibular system, hair cells, supporting cells, and associated neurons. This sequence gives developmental biologists a framework for relating early tissue patterning to the later organization of auditory structures.
Genetic signals and interactions between developing tissues help establish auditory structure and function. In murine auditory organs, these influences can be examined as the otic vesicle grows and differentiates into distinct sensory and neural components. Focusing on these relationships helps investigators connect developmental instructions with the emergence of organized hearing-related anatomy.
Patterned growth matters because the otic vesicle does not simply enlarge; it gives rise to specialized regions with different roles. The cochlea, vestibular system, hair cells, supporting cells, and associated neurons must be organized as distinct components. Studying this patterning clarifies how developmental processes generate both auditory and vestibular structures within the ear.
Mouse models are valuable because they make developmental changes in auditory structures accessible in a vertebrate system. Studies can use them to investigate how altered developmental processes relate to congenital deafness and hearing loss. The same model context also supports examination of sensory-cell development and the prospects for regenerative or therapeutic strategies.
A useful developmental sequence follows the transition from otic placode to otic vesicle and then examines patterned growth and differentiation. Investigators can next assess the emergence of the cochlea, vestibular system, hair cells, supporting cells, and associated neurons. Organizing observations in this order helps relate cellular outcomes to earlier developmental events.
These studies can reveal how genetic signals and tissue interactions establish auditory structure and function. They can also identify when distinct components, including sensory cells and associated neurons, emerge during development. Such outcomes provide a basis for interpreting structural organization, understanding hearing-related abnormalities, and evaluating questions about sensory-cell development.
Applications center on congenital deafness, sensory-cell development, hearing loss, and possible regenerative or therapeutic strategies. Developmental findings from mice help researchers connect the formation of specific auditory components with later questions about impaired hearing or restoration. The model therefore links basic developmental biology to investigations of hearing loss and potential intervention approaches.