Pediatric Airway Sampling
Pediatric Airway Sampling - Related Videos
05:31
Noninvasive Sampling of Mucosal Lining Fluid for the Quantification of In Vivo Upper Airway Immune-mediator Levels
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Cited by 4 •
2017
This protocol describes a noninvasive technique for the sampling of undisturbed mucosal lining fluid from the upper airways. It can be used to perform the quantification of in vivo levels of protein mediators, such as cytokines and chemokines, in subjects of all ages.
11:19
Guidelines for Elective Pediatric Fiberoptic Intubation
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Cited by 17 •
2011
We describe guidelines to perform a safe and efficient elective fiberoptic intubation in pediatric patients while maintaining spontaneous ventilation.
09:58
A Reversible, Non-invasive Method for Airway Resistance Measurements and Bronchoalveolar Lavage Fluid Sampling in Mice
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Cited by 10 •
2010
Repeated measurements of rodent respiratory physiology and sampling of airway inflammatory cells are desirable, but generally not feasible. Here we describe a repeatable method for orally intubating mice that permits repeated measurements of airway hyperreactivity and sampling of airway inflammatory cells.
03:37
Evaluating the Effects of Different Polishing Methods on Color Stability of Dental Restorations in Pediatric Dentistry
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Cited by 1 •
2025
Here, we evaluated the impact of various polishing methods on the color stability of compomer and composite resin restorations used in anterior restorations within pediatric dentistry. This study highlights the significance of selecting appropriate polishing methods to enhance the color stability of compomer and composite resin restorations in pediatric dentistry.
04:27
Co-culturing Glutamatergic Neurons and Pediatric High-Grade Glioma Cells
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2025
This video showcases a technique for co-culturing glutamatergic neurons and pediatric high-grade glioma (pHGG) cells within a microfluidic device. Human induced pluripotent stem cells are introduced into the microfluidic system and incubated to promote their differentiation into mature cortical glutamatergic neurons. Following this, the pHGG cells are introduced onto the mature neurons, establishing a co-culture and enabling interactions between neurons and pHGG cells.
FAQs
Age, airway anatomy, and allowable sample volume strongly influence pediatric airway sampling. Infants and older children may differ in the material that can be collected and in how specimens represent airway regions. Accounting for these variables helps researchers interpret cellular, microbial, mucus, and molecular findings as developmental or disease-related differences rather than effects of collection conditions.
The sampling site and collection method shape the information available from a specimen. Nasal or throat material can support assessment of accessible airway findings, whereas lower-airway samples may address material from deeper regions. Swabs, aspirates, lavage, and induced sputum should therefore be matched to the research question because each samples a different airway context.
In developmental biology, attention to age-related patterns can connect airway growth with respiratory outcomes. Airway samples may reveal how cellular or molecular features change during development and how early infection or inflammation responses emerge. This makes sampling useful for studying relationships between developmental state and respiratory findings, rather than treating all pediatric specimens as equivalent.
A basic workflow begins by defining the research question, selecting the relevant airway region, and choosing a compatible collection method. Investigators may use a swab, aspirate, lavage, or induced sputum according to the specimen needed and the child’s age, anatomy, and feasible sample volume. The resulting material can then be examined for cells, mucus, microorganisms, or molecular material.
Standardized sampling improves the value of comparisons across pediatric airway specimens. Accounting consistently for age, sampling location, technique, and sample volume helps distinguish biological variation from differences introduced during collection. Consistent procedures can strengthen biomarker discovery and disease monitoring by making cellular, microbial, and molecular measurements more interpretable across children or across assessments.
Researchers can apply these specimens to characterize airway development, investigate early responses to infection or inflammation, and evaluate respiratory outcomes. The same sampling framework may support biomarker discovery, disease monitoring, and assessment of emerging therapies. Its value lies in linking measurable airway material with developmental patterns and respiratory changes while maintaining attention to pediatric sampling constraints.