Dielectrophoresis Assembly
Dielectrophoresis Assembly - Related Videos
10:38
Label-free Isolation and Enrichment of Cells Through Contactless Dielectrophoresis
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Cited by 19 •
2013
Contactless dielectrophoresis (cDEP) achieves sorting and enrichment of particles via their intrinsic dielectric properties. Fluidic electrode channels replace metallic electrodes traditional to DEP, suiting cDEP to non-damaging sterile characterization and sorting of biological particles. We demonstrate how to prepare a cDEP microdevice and conduct cell characterization and sorting experiments.
11:36
Assembly, Loading, and Alignment of an Analytical Ultracentrifuge Sample Cell
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Cited by 21 •
2009
The analytical ultracentrifuge (AUC) sample cell holds sample and reference buffer and during experiments and is exposed to high vacuum and rotor speeds up to 60,000 rpm. This video will demonstrate the rigorous attention to detail necessary for assembly, loading and alignment of this very important component of an AUC experiment.
09:45
Separating Beads and Cells in Multi-channel Microfluidic Devices Using Dielectrophoresis and Laminar Flow
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Cited by 8 •
2011
Dielectrophoresis (DEP) is an effective method to manipulate cells. Printed circuit boards (PCB) can provide inexpensive, reusable and effective electrodes for contact-free cell manipulation within microfluidic devices. By combining PDMS-based microfluidic channels with coverslips on PCBs, we demonstrate bead and cell manipulation and separation within multichannel microfluidic devices.
02:41
Protein Complex Assembly
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2020
Proteins can form homomeric complexes with another unit of the same protein or heteromeric complexes with different types. Most protein complexes self-assemble spontaneously via ordered pathways, while some proteins need assembly factors that guide their proper assembly. Despite the crowded intracellular environment, proteins usually interact with their correct partners and form functional complexes. Many viruses self-assemble into a fully functional unit using the infected host cell to...
10:08
Light-Induced Dielectrophoresis for Characterizing the Electrical Behavior of Human Mesenchymal Stem Cells
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Cited by 1 •
2023
Here, we present light-induced dielectrophoresis as a label-free approach for characterizing heterogeneous cell lines, specifically human mesenchymal stem cells (hMSCs). This paper describes a protocol for using and optimizing a microfluidic device with a photoconductive layer to characterize the electrical behavior of hMSCs without altering their native state.
FAQs
The direction of particle motion depends on the interaction between the induced dipole and the surrounding electric field. A particle can move toward stronger-field regions or away from them according to its electrical properties relative to the medium. Adjusting the alternating-current frequency changes this response, allowing selective positioning of particles or cells within the same assembly system.
Electrode geometry shapes the nonuniformity of the electric field, which determines where dielectrophoretic forces become stronger or weaker. Carefully designed electrode arrangements can therefore create preferred locations for particles, cells, or microscopic components. In engineering systems, this spatial control supports reproducible organization and helps form structures with predetermined layouts rather than random distributions.
Electrode geometry, alternating-current frequency, applied voltage, and fluid conditions are the main variables identified for controlling assembly. Together, they affect the field experienced by each particle and its interaction with the surrounding medium. Researchers tune these conditions to regulate movement, positioning, and connection of microscopic components for a desired microscale arrangement.
A typical setup begins by placing the particles or cells in a suitable fluid environment near the selected electrode geometry. Researchers then apply a nonuniform alternating-current field and adjust frequency, voltage, and fluid conditions while observing the resulting organization. These controls are refined until the particles occupy the intended positions or form the required microscale connections.
Engineering applications include microfabrication, particle manipulation, sensor development, and integration of functional components into miniaturized devices. The technique is useful when a project requires controlled placement at microscopic dimensions. By organizing particles or cells into selected locations, it can help researchers construct or configure small structures that support device-level functions.
The method can produce microscale structures with controlled spatial organization and can position or connect functional components within compact systems. Such control is relevant to engineered sensors and other miniaturized devices because component placement directly supports structural integration. Its value lies in translating electrical-field adjustments into organized material arrangements at a microscopic scale.