Naked Eye Detection
Naked Eye Detection - Related Videos
09:04
Covalent Binding of Antibodies to Cellulose Paper Discs and Their Applications in Naked-eye Colorimetric Immunoassays
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Cited by 14 •
2016
In this manuscript, periodate oxidation and glutaraldehyde cross-linking methods for covalent immobilization of antibodies on paper discs are presented. The binding activity of immobilized antibodies was evaluated. Based on these results, a glutaraldehyde cross-linking method was used to develop a novel paper-based immunoassay for immunoglobulin G (IgG) detection.
08:31
Optimized Griess Reaction for UV-Vis and Naked-eye Determination of Anti-malarial Primaquine
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2019
This protocol describes a novel colorimetric method for antimalarial primaquine (PMQ) detection in synthetic urines and human serums.
06:19
Eye Tracking in Cognitive Experiments
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2023
Eye tracking as the name suggests involves tracking of eye-movements. It is a non-invasive, sensitive tool that quantifies and measures eye-movements to describe an individuals' cognitive state. An eye-movement between two fixation points is called a saccade, which is one of the fastest motor movements in our body. By observing the profiles of these eye movements, scientists can better understand neural deficits in patients with cognitive impairments. In this video, we will first look at an...
08:06
Eye Movement Monitoring of Memory
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Cited by 10 •
2010
Eye movement monitoring (or eye tracking) reveals where in space the eyes linger, when and for how long. Here, we demonstrate how eye tracking can be used to investigate the integrity of memory in multiple participant populations, without requiring verbal, or otherwise explicit, reports.
09:03
Trabecular Meshwork Response to Pressure Elevation in the Living Human Eye
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Cited by 7 •
2015
Trabecular meshwork (TM) migration into Schlemm’s canal space can be induced by acute pressure elevation by ophthalmodynamometer, and observed by spectral domain optical coherence tomography. The goal of this method is to quantify the morphometric response of the living outflow tract to acute pressure elevation in living tissues in situ.
FAQs
Visibility depends on the difference producing a signal that stands out from its surroundings. In genetics, that signal may appear as an organismal appearance change, a distinct colony morphology, or a color change in an assay. The observer then uses the contrast to distinguish samples, linking a readily visible result with a possible underlying genetic difference.
The signal must be sufficiently strong and visually distinct from its background. Clear contrast improves the chance that different observers will classify a sample similarly, whereas subtle differences increase subjectivity and can escape detection. For that reason, visual assessment is best suited to unmistakable changes and should not be treated as a highly sensitive measurement.
Visual scoring provides a preliminary indication, but it does not directly establish the genetic change responsible for the observed appearance, colony form, or assay color. Its lower sensitivity and greater subjectivity can produce uncertain classifications, especially when signals are weak. Appropriate genetic or molecular methods are therefore needed to confirm an important screening result.
First, examine the organism, colony, or assay for a visible feature that contrasts with its surroundings. Next, record or classify the observed result consistently across samples, separating clear signals from ambiguous ones. Finally, follow promising or consequential observations with an appropriate genetic or molecular confirmation. This sequence uses visual inspection for speed without treating it as definitive evidence.
It can support preliminary screening for inherited traits, genetically modified organisms, and simple molecular tests when the expected result produces a readily visible signal. Colony morphology and assay color provide different kinds of observable outputs, while organismal appearance can help identify candidate phenotypes. In each case, the method narrows attention to samples requiring further confirmation.
By reducing dependence on specialized imaging equipment, visual assessment can make an initial screen faster and less equipment-intensive. Its value is greatest when many samples show clear differences that can be recognized directly. The resulting classifications remain provisional, however, so researchers should confirm selected findings with suitable genetic or molecular methods before drawing firm conclusions.