Permeable Supports
Permeable Supports - Related Videos
11:11
Canine Intestinal Organoids in a Dual-Chamber Permeable Support System
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Cited by 18 •
2022
Here, we present a protocol describing the culture of canine intestinal organoids in a dual-chamber, permeable support system. Organoid seeding in the permeable supports, the monolayer maintenance, and subsequent drug permeability experiments are described.
06:37
An Assay for Permeability of the Zebrafish Embryonic Neuroepithelium
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Cited by 11 •
2012
We describe a live whole animal quantitative measurement for permeability of the embryonic zebrafish brain. The technique analyzes the ability to retain cerebrospinal fluid and molecules of different molecular weights within the neural tube lumen and quantifies their movement out of the ventricles. This method is useful for determining differences in epithelial permeability and maturation during development and disease.
07:44
Studying the Effects of Tumor-Secreted Paracrine Ligands on Macrophage Activation using Co-Culture with Permeable Membrane Supports
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2019
Here, we present a method using permeable membrane supports to facilitate the study of non-contact paracrine signaling used by tumor cells to suppress the immune response. The system is amenable to studying the role of tumor-secreted factors in dampening macrophage activation.
07:03
An in vivo Assay to Test Blood Vessel Permeability
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Cited by 335 •
2013
We are presenting an in vivo assay to test blood vessel permeability. This assay is based on intravenous injection of a dye and subsequent visualization of its diffusion into interstitial spaces.
01:25
Permeability of Concrete
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2024
Permeability in the context of concrete refers to how easily liquids or gases can pass through the material. This quality is crucial for assessing the water-tightness and durability of concrete structures and their resistance to chemical attacks. Concrete permeability can be determined through comparative laboratory tests. These tests typically involve sealing a concrete specimen from the sides, applying water pressure to the top surface with pressure, and measuring the amount of water passing...
FAQs
Selective retention comes primarily from pore size and the experimental design. Interconnected pores can permit soluble factors, nutrients, and metabolites to move between compartments while retaining cells or microorganisms, but changing the size relationship between pores and biological entities alters what can cross. This makes the support useful for testing passage and containment rather than treating exchange as unrestricted.
These systems preserve communication without requiring the two populations to share the same compartment. Immune cells and target cells can remain separated while soluble signals move through the support, allowing investigators to examine chemokine-driven migration and inflammatory signaling in a defined arrangement. The separation helps distinguish effects associated with soluble communication from effects requiring direct cellular coexistence.
Permeable Supports can model a biological barrier by placing an interface between compartments and measuring whether relevant material crosses it. In infection studies, the retained or transmitted population may include cells or microorganisms, while soluble nutrients and metabolites continue to exchange. This combination supports analysis of barrier permeability alongside host responses, rather than measuring pathogen or immune behavior in isolation.
A basic culture workflow establishes immune and target cells in separated compartments, then uses the support to maintain exchange between them. Researchers can observe chemokine-driven migration, inflammatory signaling, or barrier permeability under the selected design conditions. The key setup decision is matching pore characteristics and compartment arrangement to whether the experiment should retain or permit passage of cells or microorganisms.
Which outcomes can be measured depends on the biological question. A model may focus on movement of immune cells toward a target, transfer of soluble inflammatory signals, passage of a pathogen, or changes in barrier permeability. Recording these outcomes in the same separated-compartment framework helps connect transport behavior with host-pathogen interaction and immune response.
Immunology and infection studies use these supports to examine host-pathogen interactions under defined conditions. They can separate immune cells from target cells while preserving exchange, making it possible to evaluate immune responses without collapsing the modeled interface. The same design can support assessment of antimicrobial treatments or strategies intended to strengthen biological barriers, linking experimental transport with intervention outcomes.