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Studying the Effects of Tumor-Secreted Paracrine Ligands on Macrophage Activation using Co-Culture with Permeable Membrane Supports
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Cancer Research
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JoVE Journal Cancer Research
Studying the Effects of Tumor-Secreted Paracrine Ligands on Macrophage Activation using Co-Culture with Permeable Membrane Supports

Studying the Effects of Tumor-Secreted Paracrine Ligands on Macrophage Activation using Co-Culture with Permeable Membrane Supports

DOI:
10.3791/60453-v

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07:44 min

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November 28, 2019

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, ,
1Lineberger Comprehensive Cancer Center,University of North Carolina at Chapel Hill, 2O’Neal Comprehensive Cancer Center,University of Alabama at Birmingham

Chapters

  • 00:04Title
  • 01:00Macrophage Culture
  • 01:32Co-culture of Tumor Cells with Macrophages
  • 02:56Treatment of Co-cultured Cells
  • 03:52Downstream Analysis of Co-cultured Cells
  • 04:38Results: The Effects of Tumor-secreted Paracrine Ligands on Macrophage Activation
  • 07:12Conclusion

Summary

Automatic Translation

Automatic Translation

Here, we present a method using permeable membrane supports to facilitate the study of non-contact paracrine signaling used by tumor cells to suppress the immune response. The system is amenable to studying the role of tumor-secreted factors in dampening macrophage activation.

Tags

Keywords: Tumor-secreted Paracrine LigandsMacrophage ActivationCo-culturePermeable MembraneImmune SuppressionParacrine SignalingCell-to-cell ContactMolecular Biological TechniquesPeritoneal MacrophagesTumor CellsDMEMInterferon Gamma

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