Ph-sensitive Reporter
Ph-sensitive Reporter - Related Videos
09:13
Sensitive Measurement of Mitophagy by Flow Cytometry Using the pH-dependent Fluorescent Reporter mt-Keima
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Cited by 33 •
2018
Mitophagy, the selective degradation of mitochondria, has been implicated in mitochondrial homeostasis and is deregulated in various human diseases. However, convenient experimental methods for measuring mitophagy activity are very limited. Here, we provide a sensitive assay for measuring mitophagy activity using flow cytometry.
03:11
Bioreporter Assay: A Sensitive Technique using a Bioluminescent Reporter System to Detect SARS-CoV-2 Antibodies
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2025
In this video, we show the HiBit-RBD bioreporter system-based assay for detecting SARS-CoV-2 neutralizing antibodies. This system targets the viral RBD domain by using a bioluminescent reporter system containing HiBiT fused to the RBD domain.
01:24
Data Reporting and Recording
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2023
Reporting and recording are crucial in data documentation. The timely, thorough, and accurate documentation of facts is essential when recording patient data. Failure to record findings during an assessment or interpretation of a problem will result in loss of information and make the patient document unreliable. The reader is left with general impressions if the information is not specific. A recording is documenting data of the individual's health information in a traceable, secure, and...
10:30
Dye-sensitized Solar Cells
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2023
Source: Tamara M. Powers, Department of Chemistry, Texas A&M University Today's modern world requires the use of a large amount of energy. While we harness energy from fossil fuels such as coal and oil, these sources are nonrenewable and thus the supply is limited. To maintain our global lifestyle, we must extract energy from renewable sources. The most promising renewable source, in terms of abundance, is the sun, which provides us with more than enough solar energy to fully fuel our...
03:50
A Lentiviral Transcriptional Reporter System to Generate a Glioblastoma Stem Cell Differentiation Reporter Line
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2025
This video demonstrates a technique to generate a glioblastoma stem cell reporter line to identify the differentiation of glioblastoma stem cells (GSCs) into astroglia. It outlines the steps involved in seeding GSCs, transducing them with a lentivirus encoding GFP, and determining GFP reporter expression through flow cytometry.
FAQs
The key molecular event is pH-dependent protonation of the reporter. As acidity changes, protonation alters the chromophore’s ionization state, which can modify either fluorescence intensity or spectral properties. These signal changes allow researchers to follow local pH dynamics rather than relying only on endpoint measurements, making the reporter useful for observing changing conditions within cells and organelles.
Changes in fluorescence intensity indicate that acidity has altered the reporter’s detectable output, whereas spectral changes describe shifts in the wavelengths or overall profile of that signal. Both arise from altered chromophore ionization, but they offer different ways to examine pH behavior. This distinction helps researchers select the signal feature most informative for a particular cellular or organelle-based measurement.
Endosomes and phagosomes are especially informative because their acidification is linked to intracellular trafficking and immune-cell activity. Measuring reporter signals in these compartments can connect local acidity with pathogen entry, movement through the cell, and antimicrobial processes. The same approach can also examine other biological environments where pH changes accompany cellular responses or host-pathogen interactions.
Researchers can follow signal changes over time to determine how local acidity changes as a pathogen enters a cell and moves through intracellular compartments. These observations help relate trafficking-associated acidification to pathogen survival and to the responses of infected or immune cells. The approach is particularly valuable because it links spatially local conditions with dynamic events rather than treating infection as a single endpoint.
A basic workflow is to monitor the reporter signal in the relevant cells, organelles, or other biological environments, then examine how that signal changes as infection or an immune response proceeds. Researchers can compare the resulting acidity-related patterns with pathogen entry, intracellular trafficking, antimicrobial activity, or cellular responses. This produces a time-resolved view of local microenvironment changes.
Immunologists use these reporters when they need to connect acidification with the behavior of immune cells or pathogens inside them. Measurements can help assess how cells modify their microenvironment, how intracellular conditions relate to antimicrobial activity, and whether local acidity accompanies pathogen persistence or survival. Because the method is noninvasive and supports measurements over time, it can follow evolving interactions within living biological systems.