Intestinal Injury
Intestinal Injury - Related Videos
07:07
Murine Model of Intestinal Ischemia-reperfusion Injury
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Cited by 50 •
2016
Here we describe the detailed procedure of intestinal ischemia-reperfusion in mice which results in reproducible injury without mortality to encourage the standardization of this technique across the field. This model of intestinal ischemia-reperfusion injury can be utilized to study the cellular and molecular mechanisms of injury and regeneration.
07:05
Important Endpoints and Proliferative Markers to Assess Small Intestinal Injury and Adaptation using a Mouse Model of Chemotherapy-Induced Mucositis
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Cited by 9 •
2019
Here, we present a protocol to establish important endpoints and proliferative markers of small intestinal injury and compensatory hyperproliferation using a model of chemotherapy-induced mucositis. We demonstrate the detection of proliferating cells using a cell cycle specific marker and using small intestinal weight, crypt depth, and villus height as endpoints.
06:29
Rodent Model of Intestinal Ischemia-Reperfusion Injury via Occlusion of the Superior Mesenteric Artery
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Cited by 3 •
2023
We describe how to generate a widely used surgical model of intestinal ischemia-reperfusion injury (IRI) in rodents. The procedure involves occlusion of the superior mesenteric artery followed by the restoration of blood flow. This model is useful for studies investigating occlusive causes of intestinal IRI in both veterinary and human medicine.
08:55
Intestinal Stem Cell Isolation and Culture in a Porcine Model of Segmental Small Intestinal Ischemia
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Cited by 15 •
2018
This protocol will enable readers to successfully establish a porcine model of segmental intestinal ischemia and subsequently isolate and culture intestinal stem cells for the study of epithelial repair following injury.
10:58
A Protocol for Lentiviral Transduction and Downstream Analysis of Intestinal Organoids
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Cited by 81 •
2015
In this video protocol we give a step by step explanation of lentiviral transduction in organoids of primary intestinal epithelium and of processing and downstream analysis of these cultures by quantitative RT-PCR, RNA-microarray and immunohistochemistry.
FAQs
Damage to the intestinal epithelium can weaken the barrier that separates gut contents from surrounding tissues. This disruption may promote fluid loss and change how intestinal microbes interact with immune cells. Consequently, the injury can affect local tissue responses as well as the intestine’s ability to maintain normal digestive and absorptive functions.
Inflammation, infection, ischemia, mechanical trauma, and toxic exposure can damage intestinal structure or function through different initiating processes. The resulting pattern may therefore vary in how strongly it affects the epithelial barrier, digestion, absorption, or interactions among microbes and immune cells. Identifying the likely cause helps frame subsequent medical assessment.
The intestine must preserve both functional tissue and a protective epithelial boundary. When injury disrupts these features, digestion and absorption may become less effective, while altered microbial contact with immune cells can influence tissue responses. These linked effects explain why intestinal injury is relevant not only to gastrointestinal function but also to barrier and immune biology.
Assessment focuses on identifying damage to intestinal structure or function and determining its clinical context. This information can support diagnosis and management when intestinal injury is associated with inflammatory bowel disease, intestinal obstruction, or treatment-related toxicity. Evaluation is therefore useful for connecting observed intestinal problems with an underlying disease process or exposure.
Intestinal injury is particularly important in the context of inflammatory bowel disease, intestinal obstruction, and toxicity related to treatment. In these settings, recognizing impaired intestinal structure or function can help inform diagnosis and management. The same assessment may also clarify how disrupted barrier integrity contributes to symptoms or complications associated with the underlying condition.
Research examines epithelial repair, barrier integrity, and tissue regeneration as potential routes to better care. These areas may support development of biomarkers for detecting or characterizing injury, therapies aimed at limiting damage or promoting repair, and recovery strategies that restore intestinal function. The goal is to translate knowledge of tissue responses into improved clinical management.