Lung Histopathology
Lung Histopathology - Related Videos
04:10
Non-Invasive Endotracheal Administration of Lipopolysaccharide to Induce Acute Lung Injury in Rodents
0 Views •
2025
In this study, a non-invasive endotracheal instillation technique is demonstrated to develop ALI in rats using a basic laryngoscope to ensure precise LPS delivery to the lungs. The LPS is instilled via an endotracheal tube using a laryngoscope. The magnitude of lung injury is evaluated at multiple time points.
04:42
Picrosirius Red Staining for Semiquantitative Histopathologic Evaluation of Collagen Deposition in Murine Models of Chronic Lung Allograft Rejection
0 Views •
Cited by 1 •
2025
Picrosirius Red staining is a semiquantitative method for objectively assessing collagen deposition in murine pulmonary fibrotic remodeling in a spatially resolved manner. Apart from evaluating total collagen deposition, Picrosirius Red staining allows for differentiating collagen fibers of different thicknesses.
07:06
A Simple Method of Mouse Lung Intubation
0 Views •
Cited by 50 •
2013
This paper describes a striaghforward and efficient method of intubating mice for pulmonary function measurements or pulmonary instillation, that allows the mice to recover and be studied at later times. The procedure involves an inexpensive fiberoptic light source that directly illuminates the trachea.
14:21
Optical Frequency Domain Imaging of Ex vivo Pulmonary Resection Specimens: Obtaining One to One Image to Histopathology Correlation
0 Views •
Cited by 21 •
2013
A method to image ex vivo pulmonary resection specimens with optical frequency domain imaging (OFDI) and obtain precise correlation to histology is described, which is essential to developing specific OFDI interpretation criteria for pulmonary pathology. This method is applicable to other tissue types and imaging techniques to obtain precise imaging to histology correlation for accurate image interpretation and assessment. Imaging criteria established with this technique would then be...
03:06
Preparation of a Murine Oropharyngeal Aspiration Model to Establish Bacterial Lung Infections
0 Views •
2026
Source: Nielsen, T. B., et al., Murine Oropharyngeal Aspiration Model of Ventilator-associated and Hospital-acquired Bacterial Pneumonia. J. Vis. Exp. (2018)This video demonstrates the procedure of delivering bacteria through the oropharynx into the lungs of an anesthetized mouse to induce lung infection and trigger an immune response.
FAQs
Alveoli, airways, blood vessels, and the interstitium each reveal different aspects of pulmonary disease. Examining these compartments alongside inflammatory or neoplastic cells helps connect the location and pattern of tissue change with processes such as infection, injury, fibrosis, emphysema, or tumor development. This compartment-based approach supports more precise disease classification than examining isolated cellular features alone.
Hematoxylin and eosin staining provides the routine structural view used to assess lung sections. It allows examination of tissue organization, alveolar and airway changes, vascular structures, interstitium, and inflammatory or neoplastic cells. When this routine view does not fully clarify tissue composition or disease-related features, specialized stains or immunohistochemistry can provide additional information.
Cellular patterns help link microscopic observations to the mechanisms underlying disease or injury. The distribution and character of inflammatory or neoplastic cells, together with changes in surrounding tissue compartments, can indicate how a process affects the lung. Interpreting these relationships is useful for distinguishing disease categories, assessing progression, and identifying features relevant to treatment decisions.
Preparation begins with tissue fixation, followed by sectioning and staining, typically with hematoxylin and eosin. The resulting sections are examined for changes in alveoli, airways, blood vessels, interstitium, and associated cells. This sequence preserves tissue structure and produces a consistent microscopic view that can be supplemented by specialized stains or immunohistochemistry when needed.
Specialized stains and immunohistochemistry are useful when routine hematoxylin and eosin examination does not fully resolve tissue composition or clinically relevant features. These approaches can clarify particular components or biomarkers within a specimen. In medical evaluation, the added information may support disease classification, improve characterization of lung tumors, and inform treatment planning.
In medicine, microscopic findings contribute to diagnosis and classification across conditions including pneumonia, fibrosis, emphysema, and lung tumors. The same approach can help evaluate disease progression and therapeutic responses by comparing structural and cellular changes in tissue. Quantitative image analysis extends this assessment by measuring tissue features and supporting more systematic interpretation of clinically relevant patterns.