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Optical Frequency Domain Imaging of Ex vivo Pulmonary Resection Specimens: Obtaining One to One Image to Histopathology Correlation
JoVE Journal
Bioengineering
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JoVE Journal Bioengineering
Optical Frequency Domain Imaging of Ex vivo Pulmonary Resection Specimens: Obtaining One to One Image to Histopathology Correlation

Optical Frequency Domain Imaging of Ex vivo Pulmonary Resection Specimens: Obtaining One to One Image to Histopathology Correlation

DOI:
10.3791/3855-v

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14:21 min

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January 22, 2013

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, , , , , ,
1Department of Pathology,Harvard Medical School, 2Massachusetts General Hospital, 3Wellman Center for Photomedicine,Harvard Medical School, 4Pulmonary and Critical Care Unit,Massachusetts General Hospital, 5Pulmonary and Critical Care Unit,Harvard Medical School

Chapters

  • 00:05Title
  • 03:00Imaging System Set-up
  • 03:41Tissue Preparation
  • 04:58Tissue Marking
  • 06:20Imaging of Tissue
  • 07:21Tissue Collection
  • 08:07Results: Correlation between OFDI and Histology
  • 13:45Conclusion

Summary

Automatic Translation

Automatic Translation

A method to image ex vivo pulmonary resection specimens with optical frequency domain imaging (OFDI) and obtain precise correlation to histology is described, which is essential to developing specific OFDI interpretation criteria for pulmonary pathology. This method is applicable to other tissue types and imaging techniques to obtain precise imaging to histology correlation for accurate image interpretation and assessment. Imaging criteria established with this technique would then be applicable to image assessment in future in vivo studies.

Tags

Optical Frequency Domain ImagingEx Vivo Pulmonary Resection SpecimensImage To Histopathology CorrelationLung CancerSquamous CellSmall CellAdenocarcinomasRadiological ResolutionBronchoscopic LimitationsHistology BiopsiesHigh-resolution Imaging ModalitiesOptical Frequency Domain Imaging (OFDI)3-dimensional ViewsCoronary Artery AtherosclerosisEsophageal Intestinal MetaplasiaDysplasiaBronchoscopic OCT/OFDIIn Vivo Imaging ToolPulmonary AirwaysParenchymaAnimal ModelsHuman AirwayOCT Imaging

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