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JoVE Journal
Bioengineering

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JoVE Journal Bioengineering
Optical Frequency Domain Imaging of Ex vivo Pulmonary Resection Specimens
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Optical Frequency Domain Imaging of Ex vivo Pulmonary Resection Specimens: Obtaining One to One Image to Histopathology Correlation

Article DOI: 10.3791/3855
January 22nd, 2013 •
1,2,3, 4, 1,2, 1,2, 2,3, 1,2,3, 2,3,5
1Department of Pathology, Harvard Medical School, 2Massachusetts General Hospital, 3Wellman Center for Photomedicine, Harvard Medical School, 4Pulmonary and Critical Care Unit, Massachusetts General Hospital, 5Pulmonary and Critical Care Unit, Harvard Medical School

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Summary
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January 22nd, 2013

A method to image ex vivo pulmonary resection specimens with optical frequency domain imaging (OFDI) and obtain precise correlation to histology is described, which is essential to developing specific OFDI interpretation criteria for pulmonary pathology. This method is applicable to other tissue types and imaging techniques to obtain precise imaging to histology correlation for accurate image interpretation and assessment. Imaging criteria established with this technique would then be applicable to image assessment in future in vivo studies.

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