Neonatal Cardiac Fibroblasts
Neonatal Cardiac Fibroblasts - Related Videos
08:16
Neonatal Cardiac Scaffolds: Novel Matrices for Regenerative Studies
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Cited by 3 •
2016
In these studies, we provide methodology for novel, neonatal, murine cardiac scaffolds for use in regenerative studies.
07:48
Myocardial Infarction in Neonatal Mice, A Model of Cardiac Regeneration
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Cited by 37 •
2016
This protocol describes a highly reproducible model of cardiac regeneration by surgical induction of myocardial infarction in the left ventricle of postnatal day 1 mice. The method involves induction of hypothermic anesthesia and ligation of the left anterior descending coronary artery.
07:50
Isolation and Culture of Neonatal Mouse Cardiomyocytes
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Cited by 187 •
2013
Primary mouse cardiomyocyte cultures are one of the pivotal tools for the investigation of myofibrillar organization and function. The following protocol describes the isolation and culture of primary cardiomyocytes from neonatal mouse hearts. The resulting cardiomyocyte cultures may be subsequently used for a variety of biomechanical, biochemical and cell-biological assays.
10:45
Isolation and Characterization of Adult Cardiac Fibroblasts and Myofibroblasts
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Cited by 20 •
2020
Obtaining a pure population of fibroblasts is crucial to studying their role in wound repair and fibrosis. Described here is a detailed method to isolate fibroblasts and myofibroblasts from uninjured and injured mouse hearts followed by characterization of their purity and functionality by immunofluorescence, RTPCR, fluorescence-assisted cell sorting, and collagen gel contraction.
03:58
Apical Resection Neonatal Mouse Model: A Surgical Procedure to Amputate the Ventricular Apex to Study Regenerative Potential in a Neonate Mouse Heart
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2025
In this video, we demonstrate the procedure to surgically remove the left ventricular apex of a neonatal mouse heart.
FAQs
These cells produce and remodel extracellular matrix proteins, thereby influencing the structural environment surrounding cardiac cells. When their activity increases after injury or other activating signals, collagen deposition can become more prominent and contribute to cardiac fibrosis. Studying this remodeling in culture helps researchers connect fibroblast behavior with changes in cardiac structure and pathological tissue repair.
Transforming growth factor beta can stimulate neonatal cardiac fibroblasts to adopt a myofibroblast-like state. This activation is associated with increased collagen deposition, making the pathway useful for examining how injury-related signaling may promote fibrotic remodeling. Experimental studies can therefore use this response to investigate mechanisms that shift fibroblasts from supporting repair toward potentially harmful matrix accumulation.
Communication occurs partly through secreted factors released by the fibroblasts. These signals can influence cardiomyocyte responses and provide a way to study interactions between connective-tissue cells and contractile heart cells. Examining such cell-cell signaling is important because cardiac remodeling reflects coordinated behavior among different cell types rather than fibroblast activity alone.
Cultures allow investigators to examine extracellular matrix production, collagen deposition, fibroblast activation, wound-healing behavior, inflammation, and secreted-factor signaling under controlled conditions. The model is especially useful for isolating fibroblast responses to injury-related stimulation or transforming growth factor beta, helping distinguish direct cellular effects from the more complex interactions present in intact cardiac tissue.
In drug-screening studies, these cells provide a culture model for evaluating how candidate treatments influence activation, matrix remodeling, collagen deposition, or related signaling responses. In tissue engineering, they help researchers study how early-life cardiac connective-tissue cells contribute to engineered cardiac environments. These applications can support efforts to limit pathological remodeling while understanding repair-associated behavior.
They offer an accessible system for investigating cardiac fibrosis, wound healing, inflammation, and communication between cardiac cell types. Because the cells come from newborn hearts, their responses also provide insight into how early-life cardiac cells react to stress. Findings from this model may help clarify pathological remodeling and inform strategies aimed at reducing excessive fibrotic change.