Unilateral Renal Iri
Unilateral Renal Iri - Related Videos
05:53
An Effective Mouse Model of Unilateral Renal Ischemia-Reperfusion Injury
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Cited by 7 •
2021
Renal ischemia-reperfusion injury is associated with high morbidity and mortality in hospitalized patients. Here, we present a simple and effective mouse model of unilateral renal ischemia-reperfusion injury and provide a sequential overview of representative pathological changes observed in the kidney.
14:05
A Murine Model of Irreversible and Reversible Unilateral Ureteric Obstruction
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Cited by 47 •
2014
The murine model of irreversible unilateral ureteric obstruction (UUO) is presented together with the model of reversible UUO in which the ureteric obstruction is relieved by anastomosis of the severed ureter into the bladder. These models enable the study of renal inflammation and scarring as well as tissue remodeling.
04:12
Renal Capsule Tumor Xenografting: A Technique to Generate an Experimental Animal Model to Evaluate Prostate Carcinogenesis
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2023
In this video, we demonstrate the implantation of human-derived prostate cancer tissue grafts into the renal capsule of an immunocompromised recipient mouse. This technique can be used to assess tumor progression and develop novel therapeutic strategies.
06:56
A System for Culturing Iris Pigment Epithelial Cells to Study Lens Regeneration in Newt
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Cited by 7 •
2011
In newt, the lens regenerates always from the dorsal iris by transdifferentiation of the iris pigmented epithelial cells (IPEs). Here we describe a procedure to culture dorsal and ventral newt IPE cells and their implantation to the newt eye. The implanted cells are then studied by tissue sectioning and immunohistochemistry.
11:04
Biomolecular Detection employing the Interferometric Reflectance Imaging Sensor (IRIS)
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Cited by 2 •
2011
Quantitative, high-throughput, real-time, and label-free biomolecular detection (DNA, protein, etc.) on SiO2 surfaces can be achieved using a simple interferometric technique which relies on LED illumination, minimal optical components, and a camera. The Interferometric Reflectance Imaging Sensor (IRIS) is inexpensive, simple to use, and amenable to microarray formats.
FAQs
Ischemia first deprives tubular cells of oxygen and nutrients, disrupting energy metabolism. When blood flow returns, oxidative stress, inflammatory signaling, endothelial dysfunction, and tubular injury become prominent. This sequence matters because tissue damage does not arise solely from the interruption itself; the restoration phase can amplify injury and shape subsequent renal recovery.
The uninjured contralateral kidney provides an internal comparison for the kidney exposed to ischemia and reperfusion. This paired design helps investigators distinguish injury-associated changes from broader experimental or biological variation. It is particularly useful when examining tissue responses, candidate biomarkers, or treatment effects within the same research setting.
Energy failure, oxidative stress, inflammatory signaling, endothelial dysfunction, and tubular injury represent connected processes that influence the outcome. Studying them together can reveal how an initial blood-flow disturbance develops into measurable tissue damage. These mechanisms also provide several points for investigating protective treatments and explaining why recovery may differ from progression toward chronic disease.
Researchers can compare biological findings in the injured kidney with those in the opposite kidney to search for changes associated with localized damage. Biomarker studies may focus on signals reflecting tubular injury, oxidative stress, inflammation, or endothelial dysfunction. Such comparisons help evaluate whether a candidate marker tracks renal pathophysiology or treatment-related protection.
A study establishes a period of reduced blood flow to one kidney, restores circulation, and then assesses the resulting renal response. Investigators select measurements that address tissue damage, inflammatory or oxidative mechanisms, biomarker behavior, or recovery. The opposite kidney remains available as a comparison, supporting interpretation of localized changes.
The model is useful when researchers need to investigate renal pathophysiology while retaining a comparison kidney. Applications include evaluating protective treatments, identifying biomarkers, and examining whether injured tissue recovers or progresses toward chronic disease. Its localized design also supports focused study of how ischemia-reperfusion affects tubular, vascular, and inflammatory processes.