Tissue Sampling
Tissue Sampling - Related Videos
08:48
Quantitation of γH2AX Foci in Tissue Samples
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Cited by 7 •
2010
Quantitation of DNA double-strand breaks on the basis of γH2AX foci has become an invaluable tool, particularly in radiation biology, for the evaluation of tissue radiosensitivity and effects of radiation modifying compounds. Here we demonstrate the use of an immunofluorescence assay for quantitation of γH2AX foci in tissue samples.
02:08
A Hydrophobic Tissue Clearing Method for a Rat Brain Tissue Sample
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2025
Source: Kirchner, K.N., et al., A Hydrophobic Tissue Clearing Method for Rat Brain Tissue. J. Vis. Exp. (2020).This video demonstrates the method of hydrophobic tissue clearing of a rat brain tissue sample. The sample is sequentially dehydrated in methanol, treated with a dichloromethane and methanol mixture to dissolve lipids and pigments, and transferred to dibenzyl ether for complete tissue clearing, enabling detailed imaging without thin sectioning.
03:35
Dissociating and Culturing Neurons from Hippocampal Tissue Samples
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2025
This video demonstrates the dissociation and culture of hippocampal neurons from hippocampal tissue samples using enzymatic and mechanical dissociations. The treatment with Ara-C inhibits the growth of non-neuronal cells, facilitating the selective growth of hippocampal neurons.
03:42
Solid Phase Microextraction Sampling: A Probe-based Technique for Sample Extraction from Graft Tissues
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2023
In this video, we demonstrate SPME-probe-based extraction of metabolites from a pre-harvested donor kidney. The extracted metabolites can be used to perform metabolomic and lipidomic analysis in order to assess the organ quality for transplantation.
05:07
Sampling Strategies and Processing of Biobank Tissue Samples from Porcine Biomedical Models
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Cited by 20 •
2018
The practical application and performance of methods for generation of representative tissue samples of porcine animal models for a broad spectrum of downstream analyses in biobank projects are demonstrated, including volumetry, systematic random sampling, and differential processing of tissue samples for qualitative and quantitative morphologic and molecular analyses types.
FAQs
The selected approach depends on where the target tissue is located, how large it is, and what clinicians need to learn from it. Needle aspiration, core biopsy, endoscopic biopsy, and surgical excision are among the possible methods. Matching the approach to the clinical question helps obtain a specimen suited to the intended microscopic, molecular, or other laboratory analysis.
Specimen quality depends not only on collection but also on what happens afterward. Careful preservation and handling help maintain the material needed for laboratory preparation, staining, and examination. If the specimen is not managed appropriately, its usefulness for identifying structural or molecular abnormalities may be reduced, which can weaken the reliability of subsequent medical decisions.
Microscopic examination can reveal structural abnormalities, while molecular analysis can identify molecular abnormalities in the collected material. Using these forms of laboratory analysis supports disease identification and can contribute to cancer classification, infection assessment, or evaluation of treatment response. The relevant analysis depends on the clinical question and the type of information needed from the specimen.
After collection, a specimen is preserved and prepared in the laboratory before it is stained and examined. This sequence converts the collected material into a form suitable for evaluating structural or molecular abnormalities. Maintaining quality throughout the workflow matters because the laboratory findings are used to support diagnosis and guide treatment.
Samples can provide evidence used to identify disease and classify cancer, rather than serving only as a record of abnormal tissue. Laboratory examination may assess structural or molecular abnormalities, and the resulting information can help clinicians make treatment decisions. The value of the result therefore depends on both the clinical question and preservation of specimen quality.
It can be used when clinicians need laboratory evidence related to infection or to determine how disease has responded to treatment. The sample is processed and examined for relevant abnormalities, with findings interpreted in the context of the clinical purpose. This makes sampling useful not only for initial diagnosis, but also for ongoing assessment.
Different clinical questions require different kinds of information. A specimen may be examined for microscopic structural changes, molecular abnormalities, or other laboratory findings, depending on what clinicians need to determine. Aligning analysis with the question helps ensure that the collected material produces information relevant to diagnosis, cancer classification, infection assessment, or treatment-response evaluation.