White Blood Cell Count
White Blood Cell Count - Related Videos
10:19
Using a Hemacytometer to Count Cells
0 Views •
2023
Many biomedical experiments require manipulation of a known quantity of cells, in order to achieve accurate, reproducible, and statistically-relevant data. Therefore, learning how to count cells is a particularly essential technique for any successful biomedical scientist. The most common way to count cells is by using a hemacytometer - an instrument that bears two laser-etched grids, which aid in the enumeration of an aliquot cells under a simple light microscope. This data can then be used to...
05:47
Counting and Determining the Viability of Cultured Cells
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Cited by 19 •
2008
Determining the number of cells in culture is important in standardization of culture conditions and in performing accurate quantitation experiments. In this video, we demonstrate how cells are counted using a hemacytometer.
11:01
Automated Quantification and Analysis of Cell Counting Procedures Using ImageJ Plugins
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Cited by 90 •
2016
This paper describes the quantification of hemocytometer and migration/invasion micrographs through two new open-source ImageJ plugins Cell Concentration Calculator and migration assay Counter. Furthermore, it describes image acquisition and calibration protocols as well as discusses in detail the input requirements of the plugins.
06:37
Counting Human Neural Stem Cells
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Cited by 14 •
2007
Knowledge of the exact number of viable cells is required for many tissue culture manipulations. This protocol describes how to differentiate between live and dead cells and quantify cells using a hemacytometer. Although it describes counting human neural stem/precursor cells (hNSPCs), it can be used for other cell types.
09:53
Automated Microfluidic Blood Lysis Protocol for Enrichment of Circulating Nucleated Cells
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Cited by 4 •
2009
An automated microfluidic device was developed for circulating nucleated cell enrichment from peripheral blood via erythrocyte lysis that ensures isolation of high quality sample without cell loss.
FAQs
Automated hematology analyzers quantify leukocytes by measuring physical signals produced as cells pass through the instrument. Electrical impedance detects changes associated with individual cells, while optical signals provide another way to evaluate cellular properties. These measurements allow the instrument to produce a standardized count from a blood sample and support consistent laboratory assessment.
The differential count separates major leukocyte categories, including neutrophils, lymphocytes, monocytes, eosinophils, and basophils. This adds cellular detail that a total count alone cannot provide. Examining the distribution of these cell types helps clinicians relate laboratory findings to possible infection, inflammation, immune abnormalities, or other medical conditions.
A WBC count provides a general signal of immune or inflammatory activity, but it does not identify the cause of an abnormal result on its own. Clinical symptoms, the differential count, and other laboratory findings add essential context. Combining these sources supports more meaningful evaluation than relying on the numerical result in isolation.
The blood measurement is performed with an automated hematology analyzer that assesses leukocytes through electrical impedance, optical signals, or related physical properties. When additional cellular detail is needed, the laboratory also reports a differential count for the major leukocyte types. The resulting data can then be considered with clinical findings and other tests.
Clinicians use these results when evaluating possible infection, inflammation, immune disorders, or conditions affecting bone marrow. The measurement can also be followed over time to observe disease progression or response to treatment. Its value depends on integrating the result with symptoms and complementary laboratory information rather than treating it as an isolated diagnosis.
Together, the total count and differential describe both the overall leukocyte level and the composition of the circulating leukocyte population. That combination can help characterize immune and inflammatory activity more specifically than either result alone. In clinical monitoring, repeated measurements may contribute to assessing whether a condition is changing or treatment is producing a response.