3d Spheroid Model
3d Spheroid Model - Related Videos
07:40
A 3D Spheroid Model for Glioblastoma
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Cited by 49 •
2020
Here, we describe an easy-to-use invasion assay for glioblastoma. This assay is suitable for glioblastoma stem-like cells. A Fiji macro for easy quantification of invasion, migration, and proliferation is also described.
11:34
Modeling Ovarian Cancer Multicellular Spheroid Behavior in a Dynamic 3D Peritoneal Microdevice
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Cited by 29 •
2017
To study ovarian tumor progression in a physiologically relevant model, multicellular spheroids were cultured in a microdevice under simulated fluid flow. This dynamic 3D model emulates the intraperitoneal environment with the cellular and mechanical components where ovarian cancer metastasis occurs.
04:40
Creation of Cardiac Tissue Exhibiting Mechanical Integration of Spheroids Using 3D Bioprinting
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Cited by 55 •
2017
This protocol describes 3D bioprinting of cardiac tissue without the use of biomaterials. 3D bioprinted cardiac patches exhibit mechanical integration of component spheroids and are highly promising in cardiac tissue regeneration and as 3D models of heart disease.
05:39
Extracellular Flux Assay in 3D Spheroids: A Method for Measuring the Metabolic Activity of Pancreatic Tumor Spheroids
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2023
This protocol describes the technique for performing extracellular flux assay on 3D spheroids generated from the co-culture of pancreatic cancer cells (Patu8902) and activated pancreatic fibroblasts (PS1) to measure the two major energy-producing pathways, glycolysis and mitochondrial respiration.
04:01
Spheroid Generation from Cell Lines: A Three-Dimensional (3D) Cell Culture Method
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2023
Spheroids are a type of 3D cell culture model used to more accurately represent a cell’s environment compared to 2D cell culture monolayer models. In the example protocol, we will see scientists generate spheroids from a colorectal cancer cell line and apply live-cell imaging to track the spheroids’ growth.
FAQs
Limited access to oxygen, nutrients, and metabolite clearance creates internal gradients within the structure. These gradients can produce differences between peripheral and central cells, influencing survival, differentiation, and cellular responses. In neuroscience experiments, interpreting results requires recognizing that cells may experience distinct microenvironments, which can make the model more tissue-like but also increase biological heterogeneity.
Cell-cell interactions help organize neural cells into compact structures, while extracellular-matrix signaling provides environmental cues that influence cellular behavior. Together, these signals can affect neural differentiation, tissue organization, and connectivity. Their contribution allows investigators to examine developmental or disease-related processes in a setting that captures interactions often reduced or absent in flat cultures.
A two-dimensional culture places cells on a flat surface, whereas a spheroid creates a three-dimensional arrangement with greater opportunities for cell-cell contact and spatial organization. The three-dimensional structure also supports internal gradients of oxygen, nutrients, and metabolites. Consequently, spheroids may provide more physiologically relevant responses while introducing spatial variation that is less prominent in flat cultures.
Neural cells can be assembled or maintained under conditions that limit attachment to a flat surface. This encourages cells to interact with one another and with their surrounding extracellular environment, promoting compact organization. The resulting structures can then be examined for features such as differentiation, connectivity, or cellular responses, depending on the neuroscience question being investigated.
Researchers may select this approach to study neural development, differentiation, connectivity, neurodegeneration, or responses to candidate therapies. Its value is greatest when spatial organization and interactions among neural cells are relevant to the question. Because the system remains experimentally accessible while reproducing selected tissue features, it can complement conventional cultures and contribute to studies that also include animal models.
Neural spheroids can provide information about how cells organize, differentiate, connect, or respond to candidate treatments in a three-dimensional setting. They may also reveal effects associated with internal oxygen, nutrient, and metabolite gradients. These observations help investigators assess selected features of brain tissue in vitro, while recognizing that the model complements rather than fully replaces animal studies.