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A 3D Spheroid Model for Glioblastoma
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Cancer Research
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JoVE Journal Cancer Research
A 3D Spheroid Model for Glioblastoma

A 3D Spheroid Model for Glioblastoma

DOI:
10.3791/60998-v

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07:40 min

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April 09, 2020

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, , , , , ,
1University Bordeaux, LAMC, 2INSERM U1029, University Bordeaux, 3Department of Biomedicine,University of Bergen, 4CNRS, IBGC UMR5095, 5LP2N, CNRS UMR 5298, IOA, 6Institut d’Optique Graduate School, IOA

Chapters

  • 00:05Introduction
  • 01:04Uniformly Sized Tumor Spheroid Generation
  • 02:12Invasion
  • 03:07Migration
  • 04:03Image Acquisition and Analysis
  • 04:57Results: Representative Spheroid Analyses
  • 06:49Conclusion

Summary

Automatic Translation

Automatic Translation

Here, we describe an easy-to-use invasion assay for glioblastoma. This assay is suitable for glioblastoma stem-like cells. A Fiji macro for easy quantification of invasion, migration, and proliferation is also described.

Tags

3D Spheroid ModelGlioblastoma3D Cell CultureTumor InvasionTumor MigrationCollagen MatrixMatrigelTumor HeterogeneityDrug Evaluation

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