Awake Mouse Recording
Awake Mouse Recording - Related Videos
14:06
Preparation of an Awake Mouse for Recording Neural Responses and Injecting Tracers
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Cited by 26 •
2012
Electrophysiological characterization of neuronal responses is important for understanding brain function and for guiding the placement of dyes for pathway tracing. However, many studies are performed in anesthetized animals. To understand brain function without anesthetics, we developed a method to record neuronal response properties and inject dyes in awake mouse.
09:31
Evaluating the Procedure for Performing Awake Cystometry in a Mouse Model
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Cited by 10 •
2017
This study describes the surgical procedures and experimental techniques for performing awake cystometry in a freely moving mouse. In addition, it provides experimental evidence to support its optimization and standardization.
02:54
Juxtacellular Recording and Staining for Precise Mapping of Neural Activity in an Awake Rat
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2025
Source: Moore, J. D., et al. Juxtacellular Monitoring and Localization of Single Neurons within Sub-cortical Brain Structures of Alert, Head-restrained Rats. J. Vis. Exp. (2015)This video demonstrates the method of juxtacellular recording and staining in a restrained, awake rat. The procedure involves recording signals from neurons and injecting dye to precisely localize the recording site. This technique allows accurate mapping of neural activity to the brain's anatomical structure.
10:44
Construction of Microdrive Arrays for Chronic Neural Recordings in Awake Behaving Mice
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Cited by 30 •
2013
The design and assembly of microdrives for in vivo electrophysiological recordings of brain signals from the mouse is described. By attaching microelectrode bundles to sturdy driveable carriers, these techniques allow for long-term and stable neural recordings. The lightweight design allows for unrestricted behavioral performance by the animal following drive implantation.
05:04
Echocardiography Recording in Awake Miniature Pigs
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Cited by 4 •
2023
A simple cart construct, built to perform research echocardiography in standing awake minipigs, is described, along with building considerations, training techniques, and representative ultrasound images.
FAQs
Anesthesia can broadly alter neuronal circuits and sensory processing, making it difficult to determine how recorded activity relates to conscious behavior. Recording awake animals preserves neural responses under conditions in which the mouse can perceive, learn, move, or interact with a task. This makes the resulting signals more directly relevant to relationships between brain activity and behavior.
These approaches measure different forms of neural activity. Implanted electrodes detect electrical signals, whereas fiber photometry and optical imaging detect calcium-dependent activity. Selecting among them therefore depends on the activity signal of interest and the experimental question. Their shared value is that each can link ongoing brain signals with behavioral observations in conscious mice.
Head fixation is one configuration for recording from conscious mice, while freely moving recording permits behavior without that physical restriction. Behavioral tracking can accompany either arrangement, helping researchers relate neural signals to observable actions. The selected configuration should therefore match the behavior being studied, such as movement, perception, learning, or another task-related response.
A typical workflow combines a signal-acquisition method with behavioral observation. Researchers implant electrodes or establish access for fiber photometry or optical imaging, record activity while the mouse remains conscious, and monitor behavior during the session. The animal may be head-fixed or freely moving, and behavioral tracking provides the context needed to interpret neural signals.
The approach supports studies of neural coding, perception, learning, and movement by allowing investigators to compare brain activity with behavior in real time. Because signals are collected while the mouse is conscious, researchers can examine how neural activity changes during behavioral states rather than relying only on measurements obtained under anesthesia.
Awake recordings provide a way to examine brain activity in relation to behavior under conscious conditions, making them useful for both normal and disease-focused neuroscience. Investigators can study how neural coding, perception, learning, or movement-related activity changes in neurological research and compare those patterns with activity observed in healthy animals.