Ex Vivo Erg
Ex Vivo Erg - Related Videos
09:16
Simultaneous ex vivo Functional Testing of Two Retinas by in vivo Electroretinogram System
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Cited by 36 •
2015
Ex vivo ERG can be used to record electrical activity of retinal cells directly from isolated intact retinas of animals or humans. We demonstrate here how common in vivo ERG systems can be adapted for ex vivo ERG recordings in order to dissect the electrical activity of retinal cells.
08:38
Transretinal ERG Recordings from Mouse Retina: Rod and Cone Photoresponses
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Cited by 22 •
2012
We describe a relatively simple method of transretinal electroretinogram (ERG) recordings for obtaining rod and cone photoresponses from intact mouse retina. This approach takes advantage of the block of synaptic transmission from photoreceptors to isolate their light responses and record them using field electrodes placed across the isolated flat-mounted retina.
09:10
Direct-Coupled Electroretinogram (DC-ERG) for Recording the Light-Evoked Electrical Responses of the Mouse Retinal Pigment Epithelium
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Cited by 2 •
2020
Here, we present a method for recording light-evoked electrical responses of the retinal pigment epithelium (RPE) in mice using a technique known as DC-ERGs first described by Marmorstein, Peachey, and colleagues in the early 2000s.
10:05
In vivo 19F MRI for Cell Tracking
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Cited by 21 •
2013
We describe a general protocol for in vivo cell tracking using MRI in a mouse model with ex vivo labeled cells. A typical protocol for cell labeling, image acquisition processing and quantification is included.
05:44
The c-FOS Protein Immunohistological Detection: A Useful Tool As a Marker of Central Pathways Involved in Specific Physiological Responses In Vivo and Ex Vivo
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Cited by 44 •
2016
Here, we present a protocol based on c-FOS protein immunohistological detection, a classical technique used for the identification of neuronal populations involved in specific physiological responses in vivo and ex vivo.
FAQs
The a-wave and b-wave provide distinct waveform features for relating recorded voltage changes to cellular contributions within the retina. Examining these components helps researchers move beyond a single overall response and ask which parts of visual signaling may be affected. This distinction is useful in studies of retinal degeneration, synaptic physiology, and responses to potential therapies.
The isolated retina is maintained in oxygenated physiological solution so the tissue remains under defined laboratory conditions while recordings are collected. This preparation supports electrode measurement of light-evoked voltage changes from retinal cells. Maintaining the tissue environment is therefore central to obtaining responses that can be examined for retinal function, signaling changes, or treatment effects.
Controlled flashes or patterns provide standardized visual stimuli that can be matched with the electrical responses recorded from the retina. Researchers can therefore compare waveform changes under defined light conditions rather than relying on uncontrolled illumination. This approach helps reveal how retinal signaling responds to specific stimulation and supports evaluation of functional changes across experimental conditions.
Working outside the intact eye allows retinal function to be examined in a laboratory preparation with controlled conditions. Researchers can focus on electrical activity generated by retinal tissue while assessing waveform components and stimulus responses. This makes ex vivo ERG useful for investigating visual signaling and synaptic physiology without relying solely on measurements from the complete eye.
A typical experiment uses isolated retinal tissue, places it in oxygenated physiological solution, and positions electrodes to detect voltage changes. The preparation is then exposed to controlled flashes or light patterns, and the resulting electrical activity is recorded as waveforms. Researchers analyze features such as the a-wave and b-wave to relate responses to retinal cellular contributions.
Ex vivo ERG supports studies of visual signaling, retinal degeneration, and synaptic physiology by providing measurable light-evoked electrical responses from retinal tissue. It can also be used to examine responses to potential therapies under defined experimental conditions. These applications connect waveform analysis with questions about retinal function and how disease or intervention may alter signaling.