Human Donor Eye
Human Donor Eye - Related Videos
10:22
Enrichment of Bruch's Membrane from Human Donor Eyes
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Cited by 14 •
2015
Identifying proteins specifically associated with Bruch’s membrane in human eyes is an important step in understanding the biochemical mechanisms behind eye diseases such as age-related macular degeneration. This protocol describes how to enrich this sheet of extracellular matrix for down-stream biochemical analysis.
10:14
Ex Vivo OCT-Based Multimodal Imaging of Human Donor Eyes for Research into Age-Related Macular Degeneration
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Cited by 8 •
2023
Laboratory assays can leverage prognostic value from the longitudinal optical coherence tomography (OCT)-based multimodal imaging of age-related macular degeneration (AMD). Human donor eyes with and without AMD are imaged using OCT, color, near-infrared reflectance scanning laser ophthalmoscopy, and autofluorescence at two excitation wavelengths prior to tissue sectioning.
05:18
Derivation of Leptomeninges Explant Cultures from Postmortem Human Brain Donors
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Cited by 5 •
2017
The leptomeninges explant culture protocol from human postmortem brain is a technically robust and simple way to derive fibronectin-positive meningeal fibroblasts within 6-8 weeks and cryopreserve approximately 20-30 million cells.
09:03
Trabecular Meshwork Response to Pressure Elevation in the Living Human Eye
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Cited by 7 •
2015
Trabecular meshwork (TM) migration into Schlemm’s canal space can be induced by acute pressure elevation by ophthalmodynamometer, and observed by spectral domain optical coherence tomography. The goal of this method is to quantify the morphometric response of the living outflow tract to acute pressure elevation in living tissues in situ.
08:37
Corneal Donor Tissue Preparation for Endothelial Keratoplasty
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Cited by 31 •
2012
Endothelial corneal transplantation is a surgical technique for treatment of posterior corneal diseases. Mechanical microkeratome dissection to prepare tissue results in thinner, more symmetric grafts with less endothelial cell loss and improved outcomes. Dissections can be performed at the eye bank prior to corneal transplantation surgery.
FAQs
Its main scientific value lies in preserving the relationships among the retina, optic nerve, and other associated structures in human tissue. Researchers can therefore examine how cellular organization corresponds to visual signaling and how disease-related changes affect these connected neural components. This perspective helps link laboratory observations more closely to changes that occur in human vision.
Comparing cellular organization across donor tissues allows investigators to relate structural changes to specific disorders and patterns of neuronal loss. The retina and optic nerve can be examined directly for changes associated with retinal degeneration, glaucoma, or optic nerve injury. These comparisons provide human anatomical and pathological context that may be difficult to reproduce fully in animal models or cell cultures.
Donor tissue contributes human anatomy and pathology that experimental models may not fully capture. Animal studies and cell cultures can support controlled investigation, whereas human donor eyes show how retinal and optic nerve changes are organized in the affected visual system. Using these perspectives together can strengthen interpretation of disease mechanisms and improve the clinical relevance of research findings.
The available workflow includes preserving the tissue, dissecting relevant structures, and applying histology, imaging, and molecular analysis. Dissection provides access to the retina, optic nerve, and associated regions, while histology and imaging reveal organization and pathology. Molecular analysis adds information about tissue-level changes, allowing structural observations to be related to visual signaling and disease.
Histology examines tissue organization, imaging records structural features, and molecular analysis investigates biological changes within the sampled material. Together, these approaches connect visible anatomy with cellular or molecular findings rather than relying on a single type of evidence. Their combined results can clarify how disease-associated alterations in the retina or optic nerve relate to impaired visual function.
They support investigation of retinal degeneration, glaucoma, optic nerve injury, and other disorders involving human vision. Researchers can use the tissue to study neuronal loss, examine disease-associated anatomy and pathology, and identify observations that may inform biomarker or therapeutic research. The resulting evidence helps connect laboratory findings with clinically relevant changes in visual function.