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Derivation of Leptomeninges Explant Cultures from Postmortem Human Brain Donors
 

Derivation of Leptomeninges Explant Cultures from Postmortem Human Brain Donors

Article DOI: 10.3791/55045 05:18 min January 21st, 2017
January 21st, 2017

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Summary

The leptomeninges explant culture protocol from human postmortem brain is a technically robust and simple way to derive fibronectin-positive meningeal fibroblasts within 6-8 weeks and cryopreserve approximately 20-30 million cells.

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Leptomeninges Explant Cultures Postmortem Human Brain Donors Parkinson's Disease Fibroblast Cell-based Assays Lewy Body Pathology Tissue Culture Hood Phosphate-buffered Saline Cutting Dish Sterile Forceps Blood Removal
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