Lamina Propria
Lamina Propria - Related Videos
04:52
Isolating Enteric Glial Cells From the Submucosa and Lamina Propria of a Mouse
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2025
This video demonstrates a method for isolating enteric glial cells from the submucosa and lamina propria of the mouse intestine. It outlines the steps involved in extracting submucosa and lamina propria tissue, isolating the enteric glial cells from them, and culturing the cells on a coated well-plate to establish an enteric glial cell culture.
09:48
Isolation of Lamina Propria Mononuclear Cells from Murine Colon Using Collagenase E
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Cited by 9 •
2019
The goal of this protocol is to isolate mononuclear cells that reside in the lamina propria of the colon by enzymatic digestion of the tissue using collagenase. This protocol allows for the efficient isolation of mononuclear cells resulting in a single cell suspension which in turn can be used for robust immunophenotyping.
05:01
Lamina Propria Mononuclear Cell Isolation: A Silica-based Density Gradient Centrifugation Technique to Obtain Mononuclear Cells from Murine Colon
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2023
In this video, we demonstrate the isolation of lamina propria mononuclear cells from murine colonic tissue by enzymatic digestion using collagenase E and silica-based density separation media gradient separation. The isolated mononuclear cells can be used for subsequent downstream analysis.
07:53
Isolation of Enteric Glial Cells from the Submucosa and Lamina Propria of the Adult Mouse
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Cited by 21 •
2018
Here, we describe the isolation of enteric-glial cells from the intestinal-submucosa using sequential EDTA incubations to chelate divalent cations and then incubation in non-enzymatic cell recovery solution. Plating the resultant cell suspension on poly-D-lysine and laminin results in a highly enriched culture of submucosal glial cells for functional analysis.
00:55
Laminins are the Adhesive Proteins of Basal Lamina
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2023
Laminins are heterotrimeric proteins with high molecular mass found in the extracellular matrix. Each laminin molecule is composed of three chains, viz. alpha, beta, and gamma, coded by five, four, and three paralogous genes, respectively. Laminins are categories based on the compositions of the three chains. In humans, the five forms of alpha chains are LAMA 1, LAMA 2, LAMA 3, LAMA 4, and LAMA 5. The four forms of beta chains are LAMB 1, LAMB 2, LAMB 3, and LAMB 4. The three forms of gamma...
FAQs
Rather than acting as a passive scaffold, its extracellular matrix provides structural support beneath the epithelial lining and contributes to barrier maintenance. This matrix also creates the local tissue framework in which fibroblasts, immune cells, blood vessels, and lymphatic vessels function. Studying its organization therefore connects tissue architecture with epithelial stability and local mucosal performance.
Blood and lymphatic vessels support nutrient and fluid exchange within the mucosal tissue. Their presence helps explain how the region maintains local tissue function beneath the epithelium and provides a physiological context for inflammatory signaling. In research, examining these vascular components can clarify how exchange processes and immune activity are coordinated within mucosal environments.
Fibroblasts and immune cells contribute to the lamina propria’s active signaling environment rather than serving only structural or defensive roles. Together with the extracellular matrix and vessels, they help coordinate inflammatory signaling across the epithelium. This makes the tissue relevant for investigating how local immune activity may interact with nearby sensory and enteric nerve pathways.
Its importance to neuroscience comes from its position at a mucosal interface where tissue processes can communicate with sensory and enteric nerve pathways. Studying this relationship helps connect epithelial barrier activity, fluid and nutrient exchange, immune signaling, and neural function. The resulting perspective supports research into neuroimmune interactions rather than treating mucosal tissue and nervous tissue as separate systems.
In olfaction research, the lamina propria provides a tissue context for examining communication between mucosal structures and sensory pathways. Investigators can use this perspective to relate local support, extracellular matrix organization, vascular and lymphatic components, and inflammatory signaling to sensory neurobiology. The approach is especially useful when asking how tissue-level conditions may influence mucosa-associated neural communication.
For gut research, the lamina propria offers a framework for examining interactions among epithelial tissues, vessels, fibroblasts, immune cells, and enteric nerve pathways. This supports studies of how local signaling and exchange processes relate to gut function. It also provides relevant biological context for disease research focused on neuroimmune communication within mucosal tissues.