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Isolation of Enteric Glial Cells from the Submucosa and Lamina Propria of the Adult Mouse
 

Isolation of Enteric Glial Cells from the Submucosa and Lamina Propria of the Adult Mouse

Article DOI: 10.3791/57629
August 15th, 2018

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Summary August 15th, 2018

Here, we describe the isolation of enteric-glial cells from the intestinal-submucosa using sequential EDTA incubations to chelate divalent cations and then incubation in non-enzymatic cell recovery solution. Plating the resultant cell suspension on poly-D-lysine and laminin results in a highly enriched culture of submucosal glial cells for functional analysis.

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