Monoclonal Expansion
Monoclonal Expansion - Related Videos
03:09
A Technique to Prepare a Monoclonal Bacterial Culture
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2025
Source: Lin, P., et al. Synthesizing Sodium Tungstate and Sodium Molybdate Microcapsules via Bacterial Mineral Excretion. J. Vis. Exp. (2018).This video demonstrates the preparation of a monoclonal bacterial culture for mineral production through serial dilution and colony isolation on an agar plate. By shaking the plate with glass beads, individual cells are spatially separated to form genetically uniform colonies, which are then transferred to liquid media for growth. The culture with the...
13:21
Antibody Generation: Producing Monoclonal Antibodies Using Hybridomas
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2023
Source: Frances V. Sjaastad1,2, Whitney Swanson2,3, and Thomas S. Griffith1,2,3,4 1 Microbiology, Immunology, and Cancer Biology Graduate Program, University of Minnesota, Minneapolis, MN 55455 2 Center for Immunology, University of Minnesota, Minneapolis, MN 55455 3 Department of Urology, University of Minnesota, Minneapolis, MN 55455 4 Masonic Cancer Center, University of Minnesota, Minneapolis, MN 55455 Polyclonal antibodies are defined as a collection of antibodies directed against...
10:32
Generation of Recombinant Human IgG Monoclonal Antibodies from Immortalized Sorted B Cells
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Cited by 7 •
2015
Synthesis of human monoclonal antibodies is the first step in studies aimed at unraveling the pathophysiological mechanisms of auto-antibody-mediated immune responses. We have developed a protocol to generate recombinant human immunoglobulin G (IgG) monoclonal antibodies from blood sorted B cells, including B-cell isolation, antibody cloning and in vitro synthesis.
09:57
Passive Administration of Monoclonal Antibodies Against H. capsulatum and Others Fungal Pathogens
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Cited by 8 •
2011
C57BL/6 mice have been used to study Hc pathogenesis and provide the best model. We are exploring the potential benefits of humoral immunity against this fungus and generated several mAbs [to histone H2B and a heat shock protein 60kDa] that we tested for their protective efficacy after intraperitoneal administration.
09:42
Analysis of Thermal Expansion via Dilatometry
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2023
Source: J. Jacob Chavez, Ryan T. Davis, and Taylor D. Sparks, Department of Materials Science and Engineering, The University of Utah, Salt Lake City, UT Thermal expansion is extremely important when considering which materials will be used in systems that experience fluctuations in temperature. A high or low thermal expansion in a material may or may not be desirable, depending on the application. For instance, in a common liquid thermometer, a material with a high thermal expansion would be...
FAQs
The approach follows whether descendants continue repeated mitotic division while retaining shared lineage characteristics or instead acquire differentiated neuronal or glial properties. This comparison separates self-renewal, which maintains a proliferative cell population, from differentiation, which changes the developmental state of progeny. In neuroscience, those outcomes help characterize the behavior and developmental potential of neural stem cells and progenitors.
Independent clones provide a way to examine cell-to-cell variation rather than treating all neural cells as biologically identical. If clones show different patterns of neuronal or glial development, researchers can investigate whether genetic or environmental factors contributed to those differences. Such comparisons strengthen interpretation of lineage behavior and reveal variation that a single expanded population could conceal.
Both genetic and environmental factors can influence how an expanded neural population develops. Genetic differences may produce distinct clone behaviors, while culture conditions can affect survival, repeated division, or later neuronal and glial development. Examining these influences helps researchers interpret whether an observed outcome reflects stable cellular characteristics, the surrounding conditions, or an interaction between the two.
A typical workflow begins by isolating one neural stem cell, progenitor, or engineered cell. Researchers then place it in culture conditions that support survival and repeated mitotic division, allowing descendants to multiply while retaining shared lineage characteristics. The resulting population can be evaluated for self-renewal, differentiation, and variation, depending on the experimental question.
Researchers may use this strategy for lineage tracing, disease modeling, drug screening, or preparing defined cell populations for neural repair research. Because the cells originate from a single starting cell, the expanded population supports focused analysis of cellular identity and behavior. It also allows researchers to compare how different clones respond during neural development or under experimental conditions.
Expanded clones can supply defined neural populations for examining neuronal and glial development, modeling disease-related cellular behavior, or evaluating responses relevant to drug screening. In neural repair research, producing a characterized population may support investigations of cells intended for repair-oriented studies. Comparing clones can additionally reveal variation that may affect developmental behavior or experimental interpretation.