Mouse Embryo Culture
Mouse Embryo Culture - Related Videos
07:37
Neural Tube Closure in Mouse Whole Embryo Culture
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Cited by 22 •
2011
A method allowing for direct pharmacological manipulation of mouse embryos during neurulation that bypasses maternal metabolism is described. The technique can be adapted to study different aspects of neurulation by varying the time point and pharmacological agent.
04:29
Ex Vivo Culture and Imaging of Oculomotor Slices from Transgenic Mouse Embryos
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2025
This video demonstrates the ex vivo culture and imaging of oculomotor slices from transgenic mouse embryos. The embryos are isolated and embedded in agarose. Using a vibratome, slices containing the oculomotor nuclei and eyes are obtained. The slices are maintained in culture and imaged using a fluorescence microscope.
04:47
Isolation and Culturing of Cells from Mouse Embryo Dorsolateral Telencephalons
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2025
This video demonstrates a protocol for isolating and culturing primary cerebral cortex cells from the dorsolateral telencephalon of mouse embryos.
07:57
Isolation and Culture of Oculomotor, Trochlear, and Spinal Motor Neurons from a Mouse Embryo
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2025
The video demonstrates the isolation of oculomotor, trochlear, and spinal motor neurons from a transgenic mouse embryo. The embryo expresses motor neurons labeled with a green fluorescent protein (GFP) for easy visualization and identification. The motor neurons are surgically isolated under a fluorescence microscope and maintained in a culture medium.
10:04
The Method of Rodent Whole Embryo Culture using the Rotator-type Bottle Culture System
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Cited by 21 •
2010
Whole embryo culture technique allows us to culture mouse and rat embryos ex vivo condition during limited periods corresponding to midgestation stages. In this video protocol, we demonstrate our standard procedures of rat whole embryo culture after E12.5 using the rotator-type bottle culture system.
FAQs
These conditions provide the chemical and physical environment needed for embryos to continue developmental processes outside the uterus. Nutrient-defined media supplies controlled inputs, while temperature and gas regulation helps preserve conditions compatible with cell division, tissue organization, and neural development. Maintaining these variables is essential when researchers compare developmental outcomes across experimental groups.
Neural tube formation is an early developmental event linked to the organization of the nervous system. Observing it ex vivo allows researchers to examine how neural structures emerge and whether genetic or environmental perturbations alter that process. Changes at this stage can provide evidence about mechanisms contributing to neurodevelopmental abnormalities and later brain patterning.
Embryos can be maintained under defined conditions while researchers examine developmental responses associated with a genetic or environmental change. Comparing cell division, tissue organization, neural tube formation, or neuronal differentiation across conditions helps connect a perturbation with a specific developmental outcome. This controlled comparison supports investigation of mechanisms rather than relying only on final in vivo observations.
Ex vivo culture provides direct access to developing embryos under controlled laboratory conditions, whereas in vivo development occurs within the uterus and is not observed as directly. The cultured approach therefore complements animal studies by making developmental changes easier to examine in relation to defined conditions or perturbations. It does not eliminate the need to interpret findings within normal embryonic development.
A typical workflow begins by placing developing mouse embryos into nutrient-defined culture media, followed by maintenance under controlled temperature and gas conditions. Researchers then examine developmental progression, including cell division, tissue organization, neural tube formation, brain patterning, or neuronal differentiation. The selected observation points depend on the developmental process or perturbation being investigated.
Cultured embryos can provide developmental readouts at several levels, from cell division and tissue organization to neural tube formation, brain patterning, and neuronal differentiation. These observations help researchers determine whether a condition changes a particular stage of neural development. The resulting patterns can clarify developmental mechanisms and help identify features associated with neurodevelopmental abnormalities.
This approach is useful when a candidate intervention needs to be examined during embryonic neural development under controlled conditions. Researchers can assess whether the intervention changes developmental outcomes such as neural tube formation, brain patterning, or neuronal differentiation. Such results can indicate whether an intervention modifies a developmental response and can guide further investigation of neurodevelopmental abnormalities.