T175 Flask
T175 Flask - Related Videos
08:39
Scale-Up of Mammalian Cell Culture using a New Multilayered Flask
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Cited by 4 •
2011
Cells play an instrumental and increasing role in research, and the discovery and development of new therapeutics. With this increasing need for greater number of cells we need more efficient and effective ways for growing and harvesting attachment dependent cells. A Multilayered flask with the right features can serve this purpose.
03:50
Isolation of Microparticles Derived from Apoptotic T Lymphocytes In Vitro
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2025
This video demonstrates the generation and isolation of T lymphocyte-derived microparticles, LMPs, from T lymphocytes following treatment with actinomycin-D.
07:08
Assessment of the Anticoagulant and Anti-inflammatory Properties of Endothelial Cells Using 3D Cell Culture and Non-anticoagulated Whole Blood
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Cited by 6 •
2017
We present an in vitro model which allows the study and analysis of coagulation in whole, non-anticoagulated blood. Anticoagulation in the system depends on the natural anticoagulation effect of healthy endothelial cells and endothelial cell activation will result in clotting.
07:33
In vivo Dual Substrate Bioluminescent Imaging
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Cited by 20 •
2011
Herein we describe the methods to construct, visualize, and quantify the bioluminescent reactions of both firefly and renilla luciferase enzymes expressed in metastatic breast cancer cells during their growth and metastasis in vivo.
11:13
In Vitro Permeation of FITC-loaded Ferritins Across a Rat Blood-brain Barrier: a Model to Study the Delivery of Nanoformulated Molecules
0 Views •
Cited by 15 •
2016
A method to establish an in vitro model of blood-brain barrier based on a co-culture of rat brain microvascular endothelial cells and astrocytes is described and validated. This system proved to be a valid tool to study the effect of nanoformulation on the trans-barrier permeation of fluorescent molecules.
FAQs
The treated growth surface supports attachment of adherent cells, allowing them to remain distributed across the available culture area during expansion. This attachment is important for maintaining cultures that can be monitored, fed, and collected consistently. In neuroscience experiments, stable attachment helps researchers prepare neuronal or glial populations for downstream analyses and drug-response studies.
The flask geometry provides a broad growth area, while the vented cap supports gas exchange during incubation. Together, these features help maintain an environment suitable for cell growth while allowing sterile handling. Consistent environmental conditions are especially important when cultures must remain healthy and comparable across experiments involving neuronal or glial cells.
Seeding density affects how many cells occupy the available growth surface and therefore contributes to culture consistency during expansion. If density varies between flasks, the resulting cultures may differ in their suitability for later assays. Maintaining a consistent starting density supports more reproducible preparation of cells for immunostaining, gene expression analysis, or drug-response experiments.
A reliable workflow includes introducing the adherent cells into the flask, maintaining them under controlled incubation conditions, performing consistent medium changes, and monitoring for contamination. Researchers should also keep seeding conditions consistent between cultures. These practices help preserve suitable growth conditions and produce cell material that can be used comparably across neuroscience experiments.
Researchers may use these larger-area cultures when they need to maintain or expand neuronal or glial cells before an experiment. The available growth surface can support production of sufficient cell material for planned assays rather than requiring immediate analysis from a smaller culture. This makes the format relevant to studies involving cell maintenance, expansion, and experimental preparation.
Cells expanded in a T175 Flask can provide material for immunostaining, gene expression analysis, and drug-response studies. These applications examine different aspects of the cultured population, including staining-based features, changes in gene activity, or responses to treatments. The usefulness of the resulting material depends on consistent culture conditions and effective contamination control throughout preparation.