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Assessment of the Anticoagulant and Anti-inflammatory Properties of Endothelial Cells Using 3D Cell Culture and Non-anticoagulated Whole Blood
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Assessment of the Anticoagulant and Anti-inflammatory Properties of Endothelial Cells Using 3D Cell Culture and Non-anticoagulated Whole Blood

Assessment of the Anticoagulant and Anti-inflammatory Properties of Endothelial Cells Using 3D Cell Culture and Non-anticoagulated Whole Blood

DOI:
10.3791/56227-v

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07:08 min

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September 05, 2017

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, , , , ,
1Department of Clinical Research,University of Bern, 2Graduate School for Cellular and Biomedical Sciences,University of Bern, 3Immunology Research Centre,St. Vincent’s Hospital Melbourne, 4Institute of Pathology,University of Bern, 5Institute of Molecular Animal Breeding and Biotechnology,Ludwig-Maximilian University

Chapters

  • 00:05Title
  • 00:48Coating Microcarrier Beads
  • 01:39Collecting Cells
  • 02:26Seeding Cells into the Stirrer Flask
  • 03:45Experimental Procedure
  • 04:32Incubation with Non-anticoagulated Blood
  • 05:46Results: Clotting
  • 06:28Conclusion

Summary

Automatic Translation

Automatic Translation

We present an in vitro model which allows the study and analysis of coagulation in whole, non-anticoagulated blood. Anticoagulation in the system depends on the natural anticoagulation effect of healthy endothelial cells and endothelial cell activation will result in clotting.

Tags

Keywords: 3D Cell CultureEndothelial CellsAnticoagulant PropertiesAnti-inflammatory PropertiesWhole BloodNon-anticoagulated BloodCardiovascular ResearchTransportation ResearchSurgery ResearchAnti-immunityEndothelial Cell ActivationMicrocarrier BeadsCoagulationPhosphate Buffered Saline (PBS)Dulbecco’s Modified Eagle’s Medium (DMEM)Medium 199TrypsinEDTACell Culture MediaMagnetic Stirrer Flask

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