Transwell System
Transwell System - Related Videos
08:24
The Transwell Migration Assay
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2023
Cells migration in response to chemical cues is crucial to development, immunity and disease states such as cancer. To quantify cell migration, a simple assay was developed in 1961 by Dr. Stephen Boyden, which is now known as the transwell migration assay or Boyden chamber assay. This set-up consists an insert which separates the wells of a multiwell plate into top and bottom compartments. Cells whose migration is to be studied are seeded into the top compartment and the chemoattractant...
03:20
A Transwell Tissue Model for Studying Interactions Between Human Oral Biofilms and Epithelium
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2026
Source: Alshehri, M. et. al., Organotypic Tissue Model Systems for Investigating Host-Pathogen Interactions In Vitro. J. Vis. Exp. (2025)This video demonstrates a co-culture model using multi-species oral biofilms and human oral epithelium. It highlights epithelial immune activation and enables the downstream analysis of host-pathogen interaction.
03:05
Fluorescence Based Transwell Macrophage Attraction Assay: An In Vitro Method to Study Tumor-Macrophage Interaction Through Chemical-Induced Chemotaxis
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2023
In this video, we study tumor-macrophage interaction using fluorescence-based transwell macrophage interaction assay. The assay involves the analysis of the migration of macrophages through chemical-induced chemotaxis.
07:41
Three-dimensional Cell Culture Model for Measuring the Effects of Interstitial Fluid Flow on Tumor Cell Invasion
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Cited by 13 •
2012
Interstitial fluid flow is elevated in solid tumors and can modulate tumor cell invasion. Here we describe a technique to apply interstitial fluid flow to cells embedded in a matrix and then measure its effects on cell invasion. This technique can be easily adapted to study other systems.
03:21
Establishing Villous and Decidual Organ Cultures as Ex Vivo Models of Human Maternal-Fetal Interface
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2025
This video demonstrates the establishment of primary human placental villous and decidual organ cultures in ex vivo conditions. These models are well-suited for studying pathogenesis at the human maternal-fetal interface.
FAQs
Pore size and experimental conditions determine whether cells can pass through the membrane or whether the setup primarily measures molecular transport. These variables therefore shape the biological question the assay can address. Selecting conditions that match the intended process helps distinguish cellular migration from movement of substances between compartments and supports more meaningful quantitative comparisons.
The upper and lower chambers can contain different media, chemoattractants, or target cells, creating a controlled relationship between the two compartments. A chemoattractant in the lower chamber can be used to examine cell movement toward a defined signal, while target cells provide a cellular context. This arrangement helps researchers study how environmental cues influence migration.
The extent to which substances cross the membrane provides information about molecular transport between separated compartments. In barrier-focused experiments, changes in this passage can be used to evaluate barrier integrity rather than cell movement alone. This makes the assay useful for examining how cellular or tissue barriers regulate exchange under defined experimental conditions.
A typical setup places cells or tissue in the upper chamber and adds media, a chemoattractant, or target cells to the lower chamber according to the experimental aim. Researchers then maintain the separated compartments under selected membrane and condition settings, allowing migration or molecular exchange to occur. The resulting cellular movement or barrier-related transport is measured quantitatively.
Researchers can quantify cellular movement through the membrane or assess how effectively substances pass between compartments. These measurements provide evidence about migration behavior, molecular transport, or barrier integrity, depending on the design. Interpreting the outcome requires connecting the measured change to the selected pore size, compartment contents, and other experimental conditions.
In neuroscience, the assay supports studies of neuronal and glial migration, blood-brain barrier permeability, and neuroinflammatory signaling. It can model interactions between separated cellular or tissue compartments while providing measurable movement or barrier-related outcomes. These results help investigate nervous system development, disease mechanisms, and responses to potential therapeutic interventions.