Hypothalamus Injection
Hypothalamus Injection - Related Videos
11:48
Genetic Manipulation of the Mouse Developing Hypothalamus through In utero Electroporation
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Cited by 14 •
2013
Despite the functional and medical importance of the hypothalamus, in utero genetic manipulation of its development has rarely been attempted. We show a detailed procedure for in utero electroporation into the mouse hypothalamus and show representative results of total and partial (regional) hypothalamic transfection.
01:23
Diencephalon: Hypothalamus and Coordination
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2025
The hypothalamus is a small yet highly complex and essential brain region that plays a crucial role in regulating various bodily functions. Anatomically, it is located at the base of the brain, just above the brainstem and below the thalamus, forming part of the limbic system. The hypothalamus interacts with other brain regions, including the pituitary gland, through a direct physical connection called the hypothalamic-pituitary axis. The hypothalamus receives somatic and visceral inputs and...
06:00
Cortex-, Hippocampus-, Thalamus-, Hypothalamus-, Lateral Septal Nucleus- and Striatum-specific In Utero Electroporation in the C57BL/6 Mouse
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Cited by 14 •
2016
This protocol describes in detail how to specifically transfect different regions in the C57BL/6 central nervous system via in utero electroporation. Included in this protocol are detailed instructions for transfections of regions that develop into the cortex, hippocampus, thalamus, hypothalamus, lateral septal nucleus and striatum.
08:28
Zebrafish Brain Ventricle Injection
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Cited by 72 •
2009
After neural tube formation, the neuroepithelium constricts and folds while the tube fills with embryonic cerebrospinal fluid (eCSF) to form the embryonic brain ventricles. We developed this ventricle injection technique to better visualize the fluid filled space in contrast to the neuroepithelial shape in a live embryo.
03:45
Intraductal Injection: Delivering Injection Mix into the Ducts of the Mouse Mammary Gland
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2023
This video describes an intraductal injection technique where we deliver injection mix into the ducts of mouse mammary glands.
FAQs
Stereotaxic targeting aligns a fine needle with defined hypothalamic coordinates, allowing researchers to associate an injected substance with a specific brain region. Because the deposit is localized, observed changes in physiology or behavior can be evaluated in relation to the targeted hypothalamic circuit rather than an undifferentiated brain area. This supports more precise investigation of neuronal populations and signaling pathways.
A controlled volume helps limit the substance’s spread to surrounding tissue. This matters because neighboring hypothalamic regions may participate in different functions, including feeding, stress, reproduction, sleep, or endocrine regulation. Restricting dispersion strengthens the connection between the intended target and the experimental outcome, making circuit manipulation or labeling more interpretable.
These substances provide complementary ways to study hypothalamic function. Drugs can be used to examine responses to a delivered compound, viral vectors can support manipulation of targeted neuronal populations, and tracers can help label relevant pathways or circuits. Selecting among them allows researchers to investigate signaling, connectivity, or functional consequences within defined hypothalamic regions.
Interpretation depends on the accuracy of the target coordinates, the controlled deposition of the substance, and the relationship between the targeted region and the measured outcome. When delivery remains localized, changes in physiology or behavior can be linked more confidently to the selected hypothalamic circuit or signaling pathway. These considerations are central to distinguishing regional effects from broader effects.
The procedure begins with anesthesia, followed by placement of the subject in a stereotaxic apparatus. Researchers then guide a fine needle to the selected hypothalamic coordinates and deposit a controlled volume of the chosen substance. The resulting experiment may manipulate neuronal activity, label a circuit, or examine how a targeted region contributes to physiology and behavior.
Anesthesia, a stereotaxic apparatus, a fine needle, defined target coordinates, and a controlled injection volume are central components. Together, these conditions support accurate access to a selected hypothalamic region while limiting spread into surrounding tissue. The injected material depends on the research objective and may include a drug, viral vector, tracer, or another substance.
Neuroscientists use targeted delivery when they need to connect a defined hypothalamic region or circuit with a physiological or behavioral outcome. Applications described for this approach include feeding, energy balance, stress, reproduction, sleep, and neuroendocrine regulation. By manipulating or labeling selected areas, researchers can examine how hypothalamic pathways contribute to coordinated functions.
Results can show how specific neuronal populations and signaling pathways influence physiology and behavior. Depending on the delivered substance, the experiment may emphasize functional manipulation or circuit labeling. These outcomes help relate localized hypothalamic activity to broader processes such as endocrine regulation, motivated behavior, energy balance, stress responses, reproductive function, or sleep.