Wide-bore Pipette
Wide-bore Pipette - Related Videos
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This video shows how to use a programmable puller to make patch pipettes and sharp electrodes for electrophysiology. The same procedure can be used to make a variety of glass tools, including injection needles.
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Introduction to Serological Pipettes and Pipettors
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The serological pipette is frequently used in the laboratory for transferring milliliter volumes of liquid, from less than 1 ml to up to 50 ml. The pipettes can be sterile, plastic, and disposable or sterilizable, glass and reusable. Both kinds of pipettes use a pipet-aid, for the aspiration and dispensation of liquids. Different sizes of pipettes can be used with the same pipet-aid for a variety of experimental assays. For example, serological pipettes are useful for mixing chemical solutions...
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Pressure-polishing Pipettes for Improved Patch-clamp Recording
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08:08
Odorant-induced Responses Recorded from Olfactory Receptor Neurons using the Suction Pipette Technique
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Aseptic Laboratory Techniques: Volume Transfers with Serological Pipettes and Micropipettors
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When working in a laboratory, it is imperative to minimize sources of contamination. Aseptic technique refers to procedures that permit transfer of cultures and reagents while avoiding contact with non-sterile surfaces. Serological pipettes and micropipettors are used to measure precise volumes without compromising sterility of solutions used in experiments.
FAQs
The larger opening changes how liquid and suspended material move through the tip. During aspiration and dispensing, it reduces shear forces, meaning the sliding stresses that can deform fragile material, and lowers the likelihood of clogging. This makes handling more suitable for delicate cells, tissue fragments, and viscous suspensions, especially when preserving sample structure is important.
Wide-bore Pipette performance reflects a balance between gentleness and precision. Its opening can accommodate material that might be stressed or obstruct a narrower tip, but the same design provides less precise control for very small volumes. Consequently, the tool is most useful when protecting sample integrity matters more than measuring or transferring the smallest quantities with maximum precision.
Compared with a standard-opening pipette, a wide-bore pipette favors lower mechanical stress and reduced clogging over fine-volume control. That distinction matters when a sample contains fragile cells, tissue pieces, or a viscous suspension rather than only easily handled liquid. The choice therefore depends on whether the primary challenge is protecting complex biological material or controlling a very small volume.
When transferring a delicate neural preparation, the relevant handling sequence is aspiration followed by dispensing through the wider opening, with the design supporting gentler movement of the sample. Users should also recognize the tradeoff in control: wide-bore handling can protect neurons or tissue fragments, yet it is less suited to very small-volume precision. This guides tool selection before downstream preparation.
In neuroscience, these pipettes can be used during preparation and transfer of neurons, brain tissue, and other delicate samples. The resulting material may proceed to culture, imaging, electrophysiology, or tissue-based experiments. Their value lies in supporting sample handling across several experimental formats, where damage during transfer could compromise cellular structure or viability before neural measurements begin.
The most relevant outcome is better preservation of cellular structure and viability during handling. Those improvements do not replace the downstream experiment, but they can strengthen the reliability of measurements obtained from neural samples. This is particularly important when cultures, images, electrophysiological recordings, or tissue-based analyses depend on material remaining sufficiently intact after transfer and preparation.