Encyclopedia of Experiments: Cancer Research
È necessario avere un abbonamento a JoVE per visualizzare questo Contenuto. Accedi o inizia la tua prova gratuita.
Transcript
For laser mircodissection, cytocentrifuge the entire 300 microliters of cell suspension onto a membrane-coated slide at 300 x g for 5 minutes. Then, place the membrane-coated slide on a microscope capable of laser mircodissection. Next, pipet 4.5 microliters of cell lysis master mix into the cap of the 0.2-milliliter PCR tube.
Place the cap above the sample and harvest a single cell by laser microdissection. Directly thereafter, check for isolated cells in the PCR cap. Remove the PCR tube from the laser mircodissection microscope and close the tube. Collect all the liquid at the bottom of the tube with a short spin.
