Encyclopedia of Experiments: Immunology
È necessario avere un abbonamento a JoVE per visualizzare questo Contenuto. Accedi o inizia la tua prova gratuita.
Transcript
Before performing near-infrared phototherapy of the tumor-injected mice, use a power meter to measure the light dose of a 690-nanometer wavelength laser, and adjust the output to 100 milliwatts per square centimeter. 24 hours before the treatment, intravenously inject 100 micrograms of antibody photosensitizer conjugate in 50 to 200 microliters of PBS, via the tail vein of the tumor-injected animal.
On the day of the phototherapy treatment, place the anesthetized conjugate injected tumor-laden mouse in the supine position and shield the non-target sites with aluminum foil. When all of the shields have been placed, use a 100 joules per square centimeter laser to irradiate the thoracic cavity with near-infrared light for about 30 seconds. When the irradiation is complete, and the mouse has awoken return the animal to its cage, and measure the bioluminescence daily.
