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Journal / Neuroscience / Surgical Transplantation of Mouse Neural Stem…
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Neuroscience

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JoVE Journal Neuroscience
Surgical Transplantation of Mouse Neural Stem Cells into the Spinal Cords of Mice Infected with Neurotropic Mouse Hepatitis Virus
Preparation of Human iPSC Recording Workspace and Network-Wide Recordings on High-Density Microelectrode Array (HD-MEA)
Journal (Neuroscience)
Preparation of Human iPSC Recording Workspace and Network-Wide Recordings on High-Density Microelectrode Array (HD-MEA)
Spinal Cord Electrophysiology II: Extracellular Suction Electrode Fabrication
Journal (Neuroscience)
Spinal Cord Electrophysiology II: Extracellular Suction Electrode Fabrication
 
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Surgical Transplantation of Mouse Neural Stem Cells into the Spinal Cords of Mice Infected with Neurotropic Mouse Hepatitis Virus

Article DOI: 10.3791/2834-v • 08:33 min • July 10th, 2011
July 10th, 2011 •
1,2, 1,2, 1,2, 1,2, 1,2,3
1Department of Molecular Biology and Biochemistry, University of California, Irvine, 2Sue and Bill Gross Stem Cell Center, University of California, Irvine, 3Institute for Immunology, University of California, Irvine

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The transplantation of mouse neural stem cells (NSCs) into the spinal cords of mice with established demyelination is detailed. The preparation of NSCs, the laminectomy of thoracic vertebra 9 (T9), and transplantation of NSCs is outlined along with the pre- and post-operative care of the mice.

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Surgical Transplantation Mouse Neural Stem Cells Spinal Cords Mice Infected Neurotropic Mouse Hepatitis Virus JHM Strain Demyelinating Disease Multiple Sclerosis Transplantation Of NSCs Remyelination Clinical Outcome Cell Replacement Therapies Chronic Neurologic Diseases In Vivo Models Engrafted Cells Host Tissue Microenvironment Adapted Method I) Preparation Of NSCs Ii) Pre-operative Care Of Mice Iii) Exposure Of The Spinal Cord Via Laminectomy Iv) Stereotactic Injection Of NSCs Iv) Post-operative Care
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