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Chromatin Isolation by RNA Purification (ChIRP)
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Biologia
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JoVE Journal Biologia
Chromatin Isolation by RNA Purification (ChIRP)

Chromatin Isolation by RNA Purification (ChIRP)

DOI:
10.3791/3912-v

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11:09 min

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March 25, 2012

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, ,
1Howard Hughes Medical Institute and Program in Epithelial Biology,Stanford University School of Medicine

Capitoli

  • 00:05Titolo
  • 01:25Cross-linking Cells to Preserve RNA-Chromatin Interactions
  • 02:58Cell Lysis
  • 03:37Shearing DNA by Sonication
  • 05:13Chromatin Isolation by RNA Purification (ChIRP)
  • 08:43Results: Enrichment of Target Long Noncoding RNAs by ChIRP
  • 10:34Conclusion

Summary

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ChIRP is a novel and rapid technique to map genomic binding sites of long noncoding RNAs (lncRNAs). The method takes advantage of the specificity of anti-sense tiling oligonucleotides to allow the enumeration of lncRNA-bound genomic sites.

Tags

Chromatin Isolation By RNA PurificationChIRPLong Noncoding RNAsChromatin StatesDosage CompensationImprintingDevelopmental Gene ExpressionLncRNAsChromatin Modification ComplexesPolycomb Repressive Complex 2Histone H3 Lysine 27 TrimethylationH3K27me3Gene-specific FashionCis RegulationTrans RegulationDrosophila LncRNAs RoX1RoX2X Chromosome Binding SitesHuman LncRNA HOTAIRPRC2 OccupancyGenome-wide EffectsModular ScaffoldsTERC RNATelomerase ComplexH3K4 Demethylase ComplexRNA Occupancy At Chromatin

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