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Developmental Biology

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JoVE Journal Developmental Biology
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Induction of Protein Deletion Through In Utero Electroporation to Define Deficits in Neuronal Migration in Transgenic Models

Article DOI: 10.3791/51983-v • 12:01 min • January 12th, 2015
January 12th, 2015 •
1, 1, 1, 1
1Department of Cellular and Molecular Medicine, University of Ottawa

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Neuron migration is regulated by numerous cell autonomous and non-cell autonomous factors. This protocol shows how in utero electroporation can be used to determine whether a phenotype in a transgenic mouse is due to disruption of a cell intrinsic mechanism or impairment of interaction between the neuron and its environment.

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Protein Deletion In Utero Electroporation Transgenic Models Neuronal Migration Cre-Lox System Phenotypes Cell Autonomous Mechanism Non-cell Autonomous Mechanism In Vivo Context Small Subset Of Cells Developing Cortex Brain Region Dorsal Telencephalon Medial Telencephalon Hippocampus Ganglionic Eminence Radial Migration Floxed Transgenic Mouse Line
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