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細胞外マトリックスを視覚化するために浸軟組織の電子顕微鏡をスキャンします

Laser-inflicted Injury of Zebrafish Embryonic Skeletal Muscle

Journal (Biology)

Laser-inflicted Injury of Zebrafish Embryonic Skeletal Muscle

Quantitation of Protein Expression and Co-localization Using Multiplexed Immuno-histochemical Staining and Multispectral Imaging

Journal (Biology)

Quantitation of Protein Expression and Co-localization Using Multiplexed Immuno-histochemical Staining and Multispectral Imaging

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細胞外マトリックスを視覚化するために浸軟組織の電子顕微鏡をスキャンします

Article DOI: 10.3791/54005-v • 10:21 min • June 14th, 2016

June 14th, 2016 •

Matthew K. Stephenson¹, Sean Lenihan^2,3, Roman Covarrubias^2,3, Ryan M. Huttinger^2,3, Richard J. Gumina^2,3, Douglas B. Sawyer⁴, Cristi L. Galindo^2,3

¹Department of Pathology, Microbiology, and Immunology, Vanderbilt University Medical Center, ²Department of Medicine, Vanderbilt University Medical Center, ³Division of Cardiovascular Medicine, Vanderbilt University Medical Center, ⁴Cardiovascular Institute, Maine Medical Center

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基本プロトコル号112、走査型電子顕微鏡（SEM）、心臓、細胞外マトリックス、線維症、脱細胞、心臓線維症、NaOHを浸軟

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