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Journal / Cancer Research / 从患者前列腺癌骨转移标本及其异种移植物中提取的三维（3D）有机物的建立与分析…
JoVE Journal
Cancer Research

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JoVE Journal Cancer Research
从患者前列腺癌骨转移标本及其异种移植物中提取的三维（3D）有机物的建立与分析
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从患者前列腺癌骨转移标本及其异种移植物中提取的三维（3D）有机物的建立与分析

Article DOI: 10.3791/60367-v • 07:21 min • February 3rd, 2020
February 3rd, 2020 •
1,2, 1,2, 1,2, 1,2, 1,2, 2,3, 4, 2,5, 1,2, 1,2
1Department of Urology, University of California, San Diego, 2Moores Cancer Center, University of California, San Diego, 3Department of Medicine, University of California, San Diego, 4Department of Radiation Oncology, University of California, Los Angeles, 5Department of Orthopedic Surgery, University of California, San Diego

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患者BMPC标本和骨转移性前列腺癌异种移植物的三维培养，保持其原始肿瘤的功能异质性，导致囊肿、球形和复杂的肿瘤状有机体。本手稿为异质患者衍生样本的 3D 培养及其使用 IFC 进行分析提供了优化策略和方案。

Tags

本月在JoVE， 第 156 期， 患者衍生器官 （PDO）， 旋转方法， 骨转移前列腺癌 （BMPC）， 在 Vivo 缝合成像， 免疫荧光细胞化学 （IFC）
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