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JoVE Journal Neuroscience
En gravitationsmatad transkardiell perfusionsmetod för histologisk analys av musens centrala nervsystem
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En gravitationsmatad transkardiell perfusionsmetod för histologisk analys av musens centrala nervsystem

Article DOI: 10.3791/63386-v • 10:37 min • January 21st, 2022
January 21st, 2022 •
1, 2, 1,3
1Department of Biochemistry and Molecular Biology, State University of New York Upstate Medical University, 2Department of Neurology, State University of New York Upstate Medical University, 3Department of Neuroscience and Physiology, State University of New York Upstate Medical University

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En bekväm gravitationsmatad perfusionsmetod för histologisk analys av musens centrala nervsystem presenteras. Immunofluorescerande detektion av fosforylerat α-synuklein demonstreras i en musmodell av Parkinsons sjukdom. Detta arbete beskriver också omfattande transkardiell perfusion, dissektion, vävnadsfrysning / inbäddning och frysta sektioneringssteg.

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Neurovetenskap utgåva 179
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