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JoVE Journal Biology

Real-Time Measurements of Calcium and Contractility Parameters in Human Induced Pluripotent Stem Cell-Derived Cardiomyocytes (Video) | JoVE

Assessment of Myofilament Ca2+ Sensitivity Underlying Cardiac Excitation-contraction Coupling

Journal (Biology)

Assessment of Myofilament Ca²⁺ Sensitivity Underlying Cardiac Excitation-contraction Coupling

AFM-based Mapping of the Elastic Properties of Cell Walls: at Tissue, Cellular, and Subcellular Resolutions

Journal (Biology)

AFM-based Mapping of the Elastic Properties of Cell Walls: at Tissue, Cellular, and Subcellular Resolutions

Real-Time Measurements of Calcium and Contractility Parameters in Human Induced Pluripotent Stem Cell-Derived Cardiomyocytes (Video) | JoVE

Real-Time Measurements of Calcium and Contractility Parameters in hiPSC-CMs

Article DOI: 10.3791/65326-v • 01:21 min • May 26th, 2023

May 26th, 2023 •

Rafeeh Dinani^1,2, Emmy Manders^1,2,3, Michiel Helmes^1,2,3, Lili Wang⁴, Bjorn C. Knollmann⁴, Diederik W. D. Kuster^1,2, Jolanda van der Velden^1,2

¹Division of Physiology, Amsterdam UMC, Vrije University, ²Heart Failure & Arrhythmias, Amsterdam Cardiovascular Sciences, ³CytoCypher BV, ⁴Division of Clinical Pharmacology, Vanderbilt School of Medicine

Tags

Biology Contractility Parameters Human Induced Pluripotent Stem Cell-derived Cardiomyocytes Mutation-mediated Changes Stressors Drug Interventions Platform User-friendly Reproducible Contractility Calcium Handling Pixel Correlation Intracellular Calcium Transients Calcium-sensitive Fluorophore Temperature Environment Plate Formats Data Access Cellular Pathomechanisms Compounds Functionality Preclinical Drug Screening Process HiPSC-CMs

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